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Possible involvement of maize Rop1 in the defence responses of plants to viral infection

Authors

  • YANYONG CAO,

    1. State Key Laboratory of Agro-biotechnology and Department of Plant Pathology, China Agricultural University, Beijing 100193, China
    2. Cereal Crops Institute, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China
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    • These authors contributed equally to this work.

  • YAN SHI,

    1. State Key Laboratory of Agro-biotechnology and Department of Plant Pathology, China Agricultural University, Beijing 100193, China
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    • These authors contributed equally to this work.

    • Present address: College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, China.

  • YONGQIANG LI,

    1. State Key Laboratory of Agro-biotechnology and Department of Plant Pathology, China Agricultural University, Beijing 100193, China
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  • YUQIN CHENG,

    1. State Key Laboratory of Agro-biotechnology and Department of Plant Pathology, China Agricultural University, Beijing 100193, China
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  • TAO ZHOU,

    1. State Key Laboratory of Agro-biotechnology and Department of Plant Pathology, China Agricultural University, Beijing 100193, China
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  • ZAIFENG FAN

    Corresponding author
    1. State Key Laboratory of Agro-biotechnology and Department of Plant Pathology, China Agricultural University, Beijing 100193, China
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: Email: fanzf@cau.edu.cn; fanzaifeng@126.com

SUMMARY

The expression of host genes can be altered during the process of viral infection. To investigate the viral infection-induced up-regulated gene expression changes of maize at different time intervals post-inoculation with Sugarcane mosaic virus (SCMV), a suppression subtractive hybridization cDNA library was constructed. A total of 454 cDNA clones were identified to be viral infection-induced up-regulated genes. The influence of Rop1 on the infection of maize by SCMV was investigated. The results showed that transient silencing of the ZmRop1 gene through virus-induced gene silencing enhanced the accumulation and systemic infection of SCMV and another potyvirus (Pennisetum mosaic virus) in maize plants, whereas transient over-expression of ZmRop1 in maize protoplasts reduced SCMV accumulation. Furthermore, it was demonstrated that the heterologous expression of ZmRop1 impaired Potato virus X infection in Nicotiana benthamiana plants. These data suggest that ZmRop1 may play a role in plant defence responses to viral infection.

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