Fig. S1 Symptom development and viral RNA accumulation of a coat protein (CP)-null mutant. (A) Symptoms of Nicotiana benthamiana plants and leaves inoculated with wild-type (WT) RCNMV and the CP-null mutant. Photographs were taken at 14 days post-inoculation (dpi). (B) Viral RNA accumulation in inoculated (white bars) and upper noninoculated (grey bars) leaves of N. benthamiana. Virus RNA accumulation was measured by quantitative real-time reverse transcriptase-polymerase chain reaction (qRT-PCR). Total RNA was extracted from inoculated and upper noninoculated leaves at 4 and 7 dpi, respectively. Each value is the mean and standard error relative to the WT compiled from at least three independent experiments for both RNA-1 and RNA-2 accumulation.

Table S1 Oligonucleotides used in this study.

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