These authors contributed equally to this work and should be considered as joint first authors.
Melon RNA interference (RNAi) lines silenced for Cm-eIF4E show broad virus resistance
Article first published online: 6 FEB 2012
© 2012 THE AUTHORS. MOLECULAR PLANT PATHOLOGY © 2012 BSPP AND BLACKWELL PUBLISHING LTD
Molecular Plant Pathology
Volume 13, Issue 7, pages 755–763, September 2012
How to Cite
RODRÍGUEZ-HERNÁNDEZ, A. M., GOSALVEZ, B., SEMPERE, R. N., BURGOS, L., ARANDA, M. A. and TRUNIGER, V. (2012), Melon RNA interference (RNAi) lines silenced for Cm-eIF4E show broad virus resistance. Molecular Plant Pathology, 13: 755–763. doi: 10.1111/j.1364-3703.2012.00785.x
- Issue published online: 26 JUL 2012
- Article first published online: 6 FEB 2012
Fig. S1 Construct for Cm-eIF4E silencing. (a) Sequence alignments of Cm-eIF4E (MU21698) and Cm-eIF(iso)4E (MU5578) using CLUSTAL X. The selected Cm-eIF4E sequence stretch (175 nucleotides) is shown in bold and italics, and the primer sequences that were used for the amplification of the fragment are additionally underlined; stars mark identical nucleotides. (b) Schematic representation of the intron-hairpin-structured RNA (ihp4E) expressed by the transgenic construct. The 175-nucleotide inverted complementary sequence fragments from the Cm-eIF4E gene (175-4E) form the stem, which is separated by the pdk intron.
Fig. S2Cm-eIF4E silencing in melon does not affect the susceptibility of melon to Papaya ringspot virus (PRSV), Watermelon mosaic virus (WMV), Cucumber mosaic virus (CMV) and Cucurbit aphid-borne yellows virus (CABYV). Results obtained in the analyses of plants from a segregating T2 generation inoculated with PRSV, WMV, CMV and CABYV; the relative amounts of virus RNA and Cm-eIF4E mRNA were quantified by reverse transcription-polymerase chain reaction (RT-PCR) (with the exception of CABYV agroinoculated plants, for which virus accumulation was quantified by Northern blot), the transgene-derived small interfering RNAs (siRNAs) were detected by Northern blot, and the presence of the transgene was detected by PCR.
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