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Fig. S1 Sugar contents in leaves of Solanum lycopersicum, Nicotiana benthamiana and Arabidopsis thaliana. Bars indicate means ± standard deviation. Letters above the bars indicate statistical significance; bars not sharing letters represent significant mean differences at P < 0.05 by Student's t-test.

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Fig. S2 Virulence of d-galacturonic acid catabolism-deficient mutants on Solanum lycopersicum leaves. (A) Lesion sizes of Botrytis cinerea wild-type and mutants were evaluated at 3 days post-inoculation (dpi) by determining the average lesion diameter on two composite leaves from two plants each. Data represent means ± standard deviation (n ≥ 50 independent lesions). (B) Botrytis cinerea biomass accumulation by immunological detection at 2 and 3 dpi on S. lycopersicum. Six lesion discs (30 mm in diameter) from three leaves of two plants were sampled as a pool for quantification. Data represent means ± standard deviation from two independent biological repeats. Letters above the bars indicate statistical significance; bars not sharing letters represent significant mean differences at P < 0.05 by Student's t-test.

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Fig. S3 Relative transcript levels of Bcpg1, Bcpg4 and Bcpg6 in wild-type Botrytis cinerea and d-galacturonic acid catabolism-deficient mutants during infection on Nicotiana benthamiana and Solanum lycopersicum leaves. Infected plants were sampled at 2 and 3 days post-inoculation (dpi) for RNA extraction. mRNA levels of Bcpg1, Bcpg4 and Bcpg6 genes were normalized to the levels of the constitutive reference gene Bcrpl5 and calibrated to wild-type strain B05.10 levels at time point 2 dpi on N. benthamiana leaves (set as 1), according to the 2–ΔΔCt method. Data are represented as means ± standard deviation from one biological repeat. Three technical replicates of each repeat were analysed and three independent biological repeats were performed, which showed similar results. For each time point on each plant leaf, letters above the bars indicate statistical significance; bars not sharing letters represent significant mean differences at P < 0.05 by Student's t-test.

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Fig. S4 Relative transcript levels of Arabidopsis thaliana defence-related genes during infection with Botrytis cinerea wild-type strain and d-galacturonic acid catabolism-deficient mutants. Infected leaves were sampled at 1, 2 and 3 days post-inoculation (dpi) for RNA extraction. mRNA levels of A. thaliana genes were normalized to the levels of the constitutive reference gene Atactin and calibrated to the levels with B. cinerea wild-type at time point 1 dpi (set as 1), according to the 2–ΔΔCt method. Data are represented as means ± standard deviation from one biological repeat. Three technical replicates of each repeat were analysed and three independent biological repeats were performed, which all showed similar results.

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Table S1 Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) primers of Botrytis cinerea genes used in this study.

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Table S2 Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) primers of Arabidopsis thaliana genes used in this study.

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