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mpp841-sup-0001-figureS1.doc100K

Fig. S1 Validation of microarray results using quantitative real-time polymerase chain reaction (qRT-PCR). Expression profiles of genes identified in the arrays after Phytophthora infestans inoculation of IL6-2 and M82. Left column, qRT-PCR was used to validate the microarray (right column) results.

mpp841-sup-0002-figureS2.doc139K

Fig. S2 The expression of Toll/interleukin-1 receptor–nucleotide-binding site–leucine-rich repeat (TIR-NBS-LRR) (37O19) and peroxisomal membrane protein (35P7) was reduced in both M82 and IL6-2, as determined by reverse transcription semi-quantitative polymerase chain reaction at 25 cycles of amplification. For TIR-NBS-LRR, lanes 1–4 show the silenced plants (M82, 1–3; IL6-2, 4). The nonsilenced controls are shown in lanes 5–11 (5, 6, M82 no vector; 7, 8, IL6-2 no vector; 9, 10, M82 empty vector; 11, IL6-2 empty vector). The lower panel depicts the amplification of the tomato actin gene as a loading control, amplified at 30 cycles. For the peroxisomal membrane protein, lanes 1–6 are from silenced plants (IL6-2, 1–3; M82, 4–6). Lanes 7–14 are the nonsilenced controls (7, 8, IL6-2 empty vector; 9, 10, M82 empty vector; 11, 12, IL6-2 no vector; 13, 14, M82 no vector). Again the lower panel depicts the amplification of the tomato actin gene as a loading control, amplified at 30 cycles. This figure shows the results of one trial, but results from all trials were similar.

mpp841-sup-0003-tableS1.xls698K

Table S1 Differentially expressed genes in M82 and IL6-2 in response to late blight infection in the field.

mpp841-sup-0004-tableS2.xls159K

Table S2 Genes similarly induced in M82 and IL6-2 in response to late blight infection.

mpp841-sup-0005-tableS3.xls241K

Table S3 Genes similarly repressed in M82 and IL6-2 in response to late blight infection.

mpp841-sup-0006-tableS4.xls360K

Table S4 List of genes in 10 clusters that were differentially expressed in M82 and IL6-2 in response to late blight infection.

mpp841-sup-0007-tableS5.xls43K

Table S5 Genes selected for viral-induced gene silencing (VIGS).

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