These two authors contributed equally to this work.
Gene profiling in partially resistant and susceptible near-isogenic tomatoes in response to late blight in the field
Article first published online: 6 NOV 2012
© 2012 THE AUTHORS. MOLECULAR PLANT PATHOLOGY © 2012 BSPP AND BLACKWELL PUBLISHING LTD
Molecular Plant Pathology
Volume 14, Issue 2, pages 171–184, February 2013
How to Cite
Cai, G., Restrepo, S., Myers, K., Zuluaga, P., Danies, G., Smart, C. and Fry, W. (2013), Gene profiling in partially resistant and susceptible near-isogenic tomatoes in response to late blight in the field. Molecular Plant Pathology, 14: 171–184. doi: 10.1111/j.1364-3703.2012.00841.x
- Issue published online: 2 JAN 2013
- Article first published online: 6 NOV 2012
Fig. S1 Validation of microarray results using quantitative real-time polymerase chain reaction (qRT-PCR). Expression profiles of genes identified in the arrays after Phytophthora infestans inoculation of IL6-2 and M82. Left column, qRT-PCR was used to validate the microarray (right column) results.
Fig. S2 The expression of Toll/interleukin-1 receptor–nucleotide-binding site–leucine-rich repeat (TIR-NBS-LRR) (37O19) and peroxisomal membrane protein (35P7) was reduced in both M82 and IL6-2, as determined by reverse transcription semi-quantitative polymerase chain reaction at 25 cycles of amplification. For TIR-NBS-LRR, lanes 1–4 show the silenced plants (M82, 1–3; IL6-2, 4). The nonsilenced controls are shown in lanes 5–11 (5, 6, M82 no vector; 7, 8, IL6-2 no vector; 9, 10, M82 empty vector; 11, IL6-2 empty vector). The lower panel depicts the amplification of the tomato actin gene as a loading control, amplified at 30 cycles. For the peroxisomal membrane protein, lanes 1–6 are from silenced plants (IL6-2, 1–3; M82, 4–6). Lanes 7–14 are the nonsilenced controls (7, 8, IL6-2 empty vector; 9, 10, M82 empty vector; 11, 12, IL6-2 no vector; 13, 14, M82 no vector). Again the lower panel depicts the amplification of the tomato actin gene as a loading control, amplified at 30 cycles. This figure shows the results of one trial, but results from all trials were similar.
Table S1 Differentially expressed genes in M82 and IL6-2 in response to late blight infection in the field.
Table S2 Genes similarly induced in M82 and IL6-2 in response to late blight infection.
Table S3 Genes similarly repressed in M82 and IL6-2 in response to late blight infection.
Table S4 List of genes in 10 clusters that were differentially expressed in M82 and IL6-2 in response to late blight infection.
Table S5 Genes selected for viral-induced gene silencing (VIGS).
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