The activity of Mangifera indica L. leaf extracts against the tetanus causing bacterium, Clostridium tetani

  1. Godfrey S. Bbosa1,*,
  2. Aloysius Lubega1,
  3. Nathan Musisi2,
  4. David B. Kyegombe1,
  5. Paul Waako1,
  6. Jasper Ogwal-Okeng1,
  7. Olwa Odyek1

Article first published online: 11 NOV 2007

DOI: 10.1111/j.1365-2028.2007.00858.x

Issue

African Journal of Ecology

African Journal of Ecology

Volume 45, Issue Supplement s3, pages 54–58, December 2007

Additional Information

How to Cite

Bbosa, G. S., Lubega, A., Musisi, N., Kyegombe, D. B., Waako, P., Ogwal-Okeng, J. and Odyek, O. (2007), The activity of Mangifera indica L. leaf extracts against the tetanus causing bacterium, Clostridium tetani. African Journal of Ecology, 45: 54–58. doi: 10.1111/j.1365-2028.2007.00858.x

Author Information

  1. 1

    Department of Pharmacology and Therapeutics, Faculty of Medicine, Makerere University, PO Box 7072, Kampala, Uganda

  2. 2

    Department of Veterinary Microbiology and Parasitology, Makerere University, PO Box 7062, Kampala, Uganda

* *E-mail: godfossa@yahoo.com

Publication History

  1. Issue published online: 11 NOV 2007
  2. Article first published online: 11 NOV 2007
  3. (Manuscript accepted 20 August 2007)

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Keywords:

  • Clostridium tetani;
  • minimum inhibitory concentration

Abstract

Mangifera indica L. is a common horticulture and medicinal plant, which is used traditionally to treat various infections. A previous study has shown its leaf extracts to have antibacterial activity against Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. This study investigated the activity of the leaf extracts against Clostridium tetani, which causes many deaths around the world. Ether and ethanolic leaf extracts were obtained by sequential extractions. Qualitative studies were carried out to determine the different classes of compounds in the extracts. The chemical tests showed that the ether extract had saponins, steroids and triterpenoids, while the ethanol extract had alkaloids, anthracenosides, coumarins, flavonones, reducing sugars, catechol and gallic tannins, saponins, steroids and triterpenoids. The minimum inhibitory concentration (MIC) of the extracts against the study organism was determined using the gradient serial dilution method. Gentamycin and distilled water were used as controls. Both the ethereal and ethanolic fractions showed anti-clostridium tetani activity with an MIC of 6.25 and 12.5 mg ml−1, respectively.

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