Inflammatory gene signature in ulcerative colitis with cDNA macroarray analysis
Article first published online: 22 APR 2005
Alimentary Pharmacology & Therapeutics
Volume 21, Issue 9, pages 1091–1097, May 2005
How to Cite
Okahara, S., Arimura, Y., Yabana, T., Kobayashi, K., Gotoh, A., Motoya, S., Imamura, A., Endo, T. and Imai, K. (2005), Inflammatory gene signature in ulcerative colitis with cDNA macroarray analysis. Alimentary Pharmacology & Therapeutics, 21: 1091–1097. doi: 10.1111/j.1365-2036.2005.02443.x
- Issue published online: 22 APR 2005
- Article first published online: 22 APR 2005
- Accepted for publication 23 February 2005
Background : Most array analyses of ulcerative colitis have focused on identifying susceptibility genes for ulcerative colitis.
Aim : To clarify the changes in gene expression during inflammation in ulcerative colitis colon mucosa using cDNA macroarray.
Methods : From 23 ulcerative colitis patients, 16 each of inflamed and non-inflamed specimens (total 32 samples for individual analysis) were obtained by colonoscopic biopsy. Eighteen of the 32 samples, used for pairwise analysis, consisted of nine sample pairs, each pair being from the same patient. We examined expression profiles of approximately 1300 genes with cDNA macroarray. Comparisons were made using two kinds of statistics, t-test and significance analysis of microarray in both analyses. The reproducibility of significant genes from the macroarray analysis was confirmed by real-time ploymerase chain reaction.
Results : We detected five upregulated genes, categorized into proinflammatory genes (MRP14, GROγ and SAA1) and anti-inflammatory genes (TIMP1 and Elafin) in inflamed mucosa, and one upregulated gene (L-FABP) in non-inflamed mucosa.
Conclusions : As the cDNA macroarray analysis in this study exactly reflects the total profile of gene expression in the clinical setting of ulcerative colitis, the genes identified will be directly applicable to diagnostics or as novel therapeutic targets in active ulcerative colitis.