These authors contributed equally to this work.
Impaired neutrophil chemotaxis in Crohn's disease relates to reduced production of chemokines and can be augmented by granulocyte-colony stimulating factor
Version of Record online: 25 JUL 2006
Alimentary Pharmacology & Therapeutics
Volume 24, Issue 4, pages 651–660, August 2006
How to Cite
HARBORD, M. W. N., MARKS, D. J. B., FORBES, A., BLOOM, S. L., DAY, R. M. and SEGAL, A. W. (2006), Impaired neutrophil chemotaxis in Crohn's disease relates to reduced production of chemokines and can be augmented by granulocyte-colony stimulating factor. Alimentary Pharmacology & Therapeutics, 24: 651–660. doi: 10.1111/j.1365-2036.2006.03016.x
- Issue online: 25 JUL 2006
- Version of Record online: 25 JUL 2006
- Publication data Submitted 19 April 2006 First decision 15 May 2006 Resubmitted 2 June 2006 Accepted 4 June 2006
Defective neutrophil recruitment has been described as a primary pathogenic abnormality in Crohn’s disease. Cantharidin-induced blisters provide a novel investigative tool to assess cellular influx and inflammatory mediator production during acute inflammation and allows the effects of therapy on these parameters to be measured.
To determine whether reduced neutrophil tissue penetration in Crohn's disease relates to impaired production of inflammatory mediators, and whether it can be reversed by granulocyte-colony stimulating factor (G-CSF).
Neutrophil and monocyte/macrophage populations and inflammatory mediators were measured in cantharidin blisters at 24 h. Neutrophil chemotaxis was assessed in vitro using blister fluid as the chemoattractant. The effect of s.c. G-CSF on blister phenotype was determined.
Significantly fewer neutrophils migrated into blisters in Crohn's patients. The production of neutrophil chemokines, but not other inflammatory mediators, was reduced. This significantly correlated with reduced chemotaxis in vitro. Differences were unrelated to caspase-recruitment domain 15 genotype. G-CSF significantly increased blister neutrophil concentrations in control subjects and Crohn's patients.
Reduced neutrophil migration during acute inflammation in Crohn's disease is associated with impaired production of appropriate chemoattractants. G-CSF therapy increases neutrophil tissue migration, which may partially account for its observed therapeutic effect.