Both authors contributed equally.
Low-dose aspirin reduces the gene expression of gastrokine-1 in the antral mucosa of healthy subjects
Version of Record online: 4 JUL 2008
© 2008 The Authors. Journal compilation © 2008 Blackwell Publishing Ltd
Alimentary Pharmacology & Therapeutics
Volume 28, Issue 6, pages 782–788, September 2008
How to Cite
MARTIN, G., WEX, T., TREIBER, G., MALFERTHEINER, P. and NARDONE, G. (2008), Low-dose aspirin reduces the gene expression of gastrokine-1 in the antral mucosa of healthy subjects. Alimentary Pharmacology & Therapeutics, 28: 782–788. doi: 10.1111/j.1365-2036.2008.03793.x
- Issue online: 20 AUG 2008
- Version of Record online: 4 JUL 2008
- Publication data Submitted 11 June 2008 First decision 19 June 2008 Resubmitted 30 June 2008 Accepted 30 June 2008 Epub Accepted Article 4 July 2008
Background Gastrokine 1 (GKN1), one of the most abundant transcripts in normal stomach, is down-regulated by Helicobacter pylori infection. Aspirin (ASA), which is often used for secondary prevention of cardiovascular events, can damage gastric-duodenal mucosa within 1 or 2 h of ingestion.
Aim To study the gastric mucosal expression of GKN1 during acute low-dose ASA consumption.
Methods Ten H. pylori-negative human volunteers took 100 mg ASA per day for 1 week, and underwent multiple upper GI endoscopies. GKN1 expression was analysed in antral and corpus mucosa by quantitative reverse-transcriptase polymerase chain reaction, western blot and immunohistochemistry (IHC). Gastric mucosal damage was detected endoscopically and histologically.
Results Gastrokine 1 was similarly expressed in both antral and corpus mucosa. The use of low-dose ASA led to a significant decrease (3.07 a.u. vs. 0.23 a.u., P < 0.001) in antrum at day 7, while GKN1 transcript levels in corpus mucosa were slightly elevated (twofold, P < 0.005). Western blot and IHC confirmed these changes at the protein level. Furthermore, IHC revealed a vesicular staining pattern in the cytoplasm for GKN1 that was confirmed by transfected human gastric adenocarcinoma cell line expressing GKN1.
Conclusion Our data demonstrated that low-dose ASA downregulates GKN1 expression specifically in antral mucosa.