Both authors share senior authorship and contributed equally to this work.
Peripheral blood monocyte subsets predict antiviral response in chronic hepatitis C
Article first published online: 17 AUG 2011
© 2011 Blackwell Publishing Ltd
Alimentary Pharmacology & Therapeutics
Volume 34, Issue 8, pages 960–971, October 2011
How to Cite
Rodríguez-Muñoz, Y., Martín-Vílchez, S., López-Rodríguez, R., Hernández-Bartolomé, Á., Trapero-Marugán, M., Borque, M. J., Moreno-Otero, R. and Sanz-Cameno, P. (2011), Peripheral blood monocyte subsets predict antiviral response in chronic hepatitis C. Alimentary Pharmacology & Therapeutics, 34: 960–971. doi: 10.1111/j.1365-2036.2011.04807.x
- Issue published online: 20 SEP 2011
- Article first published online: 17 AUG 2011
- Publication data , Submitted 7 April 2011, First decision 26 April 2011, Resubmitted 6 July 2011, Accepted 22 July 2011EV Pub Online 17 August 2011
Aliment Pharmacol Ther 2011; 34: 960–971
Background Hepatitis C virus infection evolves into chronic progressive liver disease in a significant percentage of patients. Monocytes constitute a diverse group of myeloid cells that mediate innate and adaptive immune response. In addition to proinflammatory CD16+ monocytes, a Tie-2+ subgroup – Tie-2 expressing monocytes (TEMs) – that has robust proangiogenic potential has been recently defined.
Aim To study the heterogeneity of peripheral blood monocytes in chronic hepatitis C (CHC) patients and to examine their proposed pathophysiological roles on disease progression and response to antiviral therapy.
Methods We studied CD16+ and Tie-2+ peripheral monocyte subpopulations in 21 healthy subjects and 39 CHC patients in various stages of disease and responses to antiviral treatment using flow cytometry. Expression profiles of proangiogenic and tissue remodelling factors in monocyte supernatants were measured using ELISA and protein arrays. Intrahepatic expression of CD14, CD31 and Tie-2 was analysed using immunofluorescence.
Results Increases of certain peripheral monocyte subsets were observed in the blood of CHC patients, wherein those cells with proinflammatory (CD16+) or proangiogenic (TEMs) potential expanded (P < 0.005, both). Notably, TEMs were significantly increased in nonresponders, particularly those with lower CD16 expression. In addition, many angiogenic factors were differentially expressed by peripheral monocytes from control or CHC patients, such as angiopoietin-1 and angiogenin (P < 0.05). Interestingly, intrahepatic TEMs were distinguished within portal infiltrates of CHC patients.
Conclusions These findings suggest for the first time the relevance of peripheral monocytes phenotypes for the achievement of response to treatment. Hence, the study of monocyte subset regulation might effect improved CHC prognoses and adjuvant therapies.