Meta-analysis: the diagnostic accuracy of lactose breath hydrogen or lactose tolerance tests for predicting the North European lactase polymorphism C/T-13910
Article first published online: 28 DEC 2011
© 2011 Blackwell Publishing Ltd
Alimentary Pharmacology & Therapeutics
Volume 35, Issue 4, pages 429–440, February 2012
How to Cite
Marton, A., Xue, X. and Szilagyi, A. (2012), Meta-analysis: the diagnostic accuracy of lactose breath hydrogen or lactose tolerance tests for predicting the North European lactase polymorphism C/T-13910. Alimentary Pharmacology & Therapeutics, 35: 429–440. doi: 10.1111/j.1365-2036.2011.04962.x
- Issue published online: 17 JAN 2012
- Article first published online: 28 DEC 2011
- Publication data Submitted 15 April 2011 First decision 1 June 2011 Resubmitted 4 December 2011 Accepted 6 December 2011 EV Pub Online 28 December 2011
Aliment Pharmacol Ther 2012; 35: 429–440
Background The diagnostic accuracy of two indirect tests of lactose digestion, lactose breath hydrogen and lactose tolerance tests, have not been systematically reviewed for comparison with available publications on genotype.
Aim To perform a meta-analysis of available studies that compares the north-European genetic polymorphism C/T-13910 with the lactose breath hydrogen and the lactose tolerance tests, to determine their ability to predict geno/phenotype relationships. We examine the effects of lactose loading dose, inclusion of children and latitudes of study centre on comparative outcome.
Methods An electronic database of the literature as well as individual references in articles were searched with the theme of genetics of lactase and comparisons with breath or lactose tolerance tests were carried out. Random effect and fixed effect models were used for breath and lactose tolerance tests respectively, to report summary accuracy measures with 95% confidence intervals (CI).
Results The search revealed 19 studies: 17 evaluated breath hydrogen, five lactose tolerance test (3/17 overlapped). Overall sensitivity was 0.88 (CI, 0.85–0.90), specificity was 0.85 (CI, 0.82–0.87) for breath test. Heterogeneity was explored by adjusting for studies including children, high or low dose lactose and to some extent by site of study. The lactose tolerance test showed sensitivity of 0.94 (0.9–0.97) and specificity of 0.90 (0.84–0.95) with a nonsignificant heterogeneity.
Conclusion The diagnostic accuracy of both tests individually reflects expected geno/phenotypes when the populations are well defined.