• Keywords:;
  • mouflon;
  • sheep;
  • Hb phenotype;
  • globins;
  • gene frequency;
  • IEF;
  • HPLC

Summary. Haemoglobin (Hb) phenotypes have been studied in 100 wild European mouflons living on the island of Sardinia by means of isoelectric focusing (pH 6.7-7.7 range) of the native tetramers, acid-urea-Triton gel-electrophoresis, and reversedphase HPLC of globin chains. The result indicates the presence of two ß-globin alleles one of which, corresponding to the ßB, being the most common (f=0.94). None were carriers of the earlier described Hb A. The new Hb was provisionally named Hb M. Severely anaemic mouflons were able to synthesize Hb C at expense of the Hb B alone, thus suggesting structural and physiological homologies between mouflon ßB and sheep ßA globin genes, and between the newly observed ßM allele and the ßB of the domestic Sardinian sheep.