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Associations of polymorphisms in the promoter I of bovine acetyl-CoA carboxylase-α gene with beef fatty acid composition

Authors

  • S. Zhang,

    1. Department of Biochemistry, Biophysics, and Molecular Biology, Ames, IA 50011, USA.
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    • Present addresses: 1Statistical Sciences North America, GlaxoSmithKline, King of Prussia, PA 19406, USA.

  • T. J. Knight,

    1. Department of Animal Science, Ames, IA 50011, USA.
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    • 2Iowa Department of Agriculture and Land Stewardship, Ankeny, IA 50023, USA.

  • J. M. Reecy,

    1. Department of Animal Science, Ames, IA 50011, USA.
    2. Center for Integrated Animal Genomics, Iowa State University, Ames, IA 50011, USA.
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  • T. L. Wheeler,

    1. United States Department of Agriculture, Agricultural Research Service, U.S. Meat Animal Research Center (USMARC), Clay Center, NE 68933, USA
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  • S. D. Shackelford,

    1. United States Department of Agriculture, Agricultural Research Service, U.S. Meat Animal Research Center (USMARC), Clay Center, NE 68933, USA
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  • L. V. Cundiff,

    1. United States Department of Agriculture, Agricultural Research Service, U.S. Meat Animal Research Center (USMARC), Clay Center, NE 68933, USA
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  • D. C. Beitz

    1. Department of Biochemistry, Biophysics, and Molecular Biology, Ames, IA 50011, USA.
    2. Department of Animal Science, Ames, IA 50011, USA.
    3. Center for Integrated Animal Genomics, Iowa State University, Ames, IA 50011, USA.
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D. C. Beitz, Department of Animal Science and Center for Integrated Animal Genomics, Iowa State University, Ames, IA 50011, USA.
E-mail: dcbeitz@iastate.edu

Summary

The objectives of this study were to identify single nucleotide polymorphisms (SNPs) in the promoter I (PI) region of the bovine acetyl-CoA carboxylase-α (ACACA) gene and to evaluate the extent to which they were associated with lipid-related traits. Eight novel SNPs were identified, which were AJ276223:g.2064T>A (SNP1), g.2155C>T (SNP2), g.2203G>T (SNP3), g.2268T>C (SNP4), g.2274G>A (SNP5), g.2340A>G (SNP6), g.2350T>C (SNP7) and g.2370A>G (SNP8). Complete linkage disequilibrium was observed among SNP1, 2, 4, 5, 6 and 8. Phenotypic data were collected from 573 cross-bred steers with six sire breeds, including Hereford, Angus, Brangus, Beefmaster, Bonsmara and Romosinuano. The genotypes of SNP1/2/4/5/6/8 were significantly associated with adjusted backfat thickness. The genotypes of SNP3 were significantly associated with triacylglycerol (TAG) content and fatty acid composition of longissimus dorsi muscle (LM) in Brangus-, Romosinuano- and Bonsmara-sired cattle. Cattle with g.2203GG genotype had greater concentrations of TAG, total lipid, total saturated fatty acid and total monounsaturated fatty acid than did cattle with g.2203GT genotype. The genotypes of SNP7 were significantly associated with fatty acid composition of LM. Cattle with genotype g.2350TC had greater amounts of several fatty acids in LM than did cattle with genotype g.2350CC. Our results suggested that the SNPs in the PI region of ACACA gene are associated with variations in the fatty acid contents in LM.

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