Present Addresses: Graduate School of Agriculture, Meiji University, Tama-ku, Kawasaki, Kanagawa 214-8571, Japan.
Differentially expressed genes during bovine intramuscular adipocyte differentiation profiled by serial analysis of gene expression
Version of Record online: 3 MAR 2010
© 2010 The Authors, Journal compilation © 2010 Stichting International Foundation for Animal Genetics
Volume 41, Issue 4, pages 436–441, August 2010
How to Cite
Mizoguchi, Y., Hirano, T., Itoh, T., Aso, H., Takasuga, A., Sugimoto, Y. and Watanabe, T. (2010), Differentially expressed genes during bovine intramuscular adipocyte differentiation profiled by serial analysis of gene expression. Animal Genetics, 41: 436–441. doi: 10.1111/j.1365-2052.2010.02022.x
- Issue online: 12 JUL 2010
- Version of Record online: 3 MAR 2010
- Accepted for publication 16 December 2009
- bovine intramuscular preadipocyte;
- marbling QTL;
- serial analysis of gene expression
Beef marbling or intramuscular fat deposition is an economically important carcass trait in Japanese Black cattle. To investigate genes involved in intramuscular adipogenesis, differential gene expression during adipogenesis in a clonal bovine intramuscular preadipocyte (BIP) cell line was profiled with serial analysis of gene expression (SAGE). We sequenced 75 283 tags for the proliferation phase (day 0) and 81 878 tags for the differentiation phase (4 days after adipogenic stimulation: day 4). A comparison of the unique SAGE tag frequencies between the day 0- and day 4-libraries revealed that 878 (2.8%) of the 30 989 unique putative transcripts were expressed at significantly different levels (P < 0.05); 401 tags (1.4%) were up-regulated and 477 tags (1.2%) were down-regulated in the day 4-library relative to the day 0-library. We confirmed up-regulation of 10 tags of the genes that were up-regulated in the previous subtraction cloning studies in BIP cells [Animal Science Journal, 76 (2005) 479]. Of the 878 differentially expressed tags, 377 were identified in the bovine RefSeq library and 356 were assigned a bovine draft genomic sequence. Fifteen tags were mapped in previously detected beef marbling quantitative trait loci (QTL) regions [Mammalian Genome, 18 (2007) 125]. These genes may be involved in the adipogenic processes of beef marbling.