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Figure S1 Two-step selection for constructing subpopulations.

Figure S2 The differences in the total allele frequency distribution at a candidate locus by using pooled populations.

Figure S3 Genomic positions of the four candidate SNPs on ECA18.

Table S1 Primer sequences for asymmetric PCR by using high-resolution melting and unlabeled probe methods using LightScanner.

Table S2 SNPs tested for the racing performance susceptibility at the candidate regions on ECA1 and ECA18.

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