These authors contributed equally.
Microarray analysis after strenuous exercise in peripheral blood mononuclear cells of endurance horses
Article first published online: 10 NOV 2010
© 2010 The Authors, Journal compilation © 2010 Stichting International Foundation for Animal Genetics
Special Issue: Horse Genomics and the Dorothy Russell Havemeyer Foundation
Volume 41, Issue Supplement s2, pages 166–175, December 2010
How to Cite
Capomaccio, S., Cappelli, K., Barrey, E., Felicetti, M., Silvestrelli, M. and Verini-Supplizi, A. (2010), Microarray analysis after strenuous exercise in peripheral blood mononuclear cells of endurance horses. Animal Genetics, 41: 166–175. doi: 10.1111/j.1365-2052.2010.02129.x
- Issue published online: 10 NOV 2010
- Article first published online: 10 NOV 2010
- Accepted for publication 20 September 2010
- gene expression;
- peripheral blood mononuclear cells (PBMCs);
It is known that moderate physical activity may have beneficial effects on health, whereas strenuous effort induces a state resembling inflammation. The molecular mechanisms underlying the cellular response to exercise remain unclear, although it is clear that the immune system plays a key role. It has been hypothesized that the physio-pathological condition that develops in athletes subjected to heavy training is caused by derangement of cellular immune regulation. The purpose of the present study was to obtain information on endurance horse gene transcription under strenuous conditions and to identify candidate genes causing immune system derangement. We performed a wide gene expression scan, using microarray technology, on peripheral blood mononuclear cells of ten horses chosen from high-level participants in national and international endurance races. The use of three different timepoints revealed changes in gene expression when post-effort samples (T1, taken immediately after the race; and T2, taken 24 h after the race) were compared with basal sample (T0, at rest). Statistical analysis showed no differences in gene expression between T0 and T2 samples, indicating complete restoration of homeostasis by 24 h after racing, whereas T1 showed strong modulation of expression, affecting 132 genes (97 upregulated, 35 downregulated). Ingenuity pathway analysis revealed that the main mechanisms and biofunctions involved were significantly associated with immunological and inflammatory responses. Real-time PCR was performed on 26 gene products to validate the array data.