*These authors contributed equally.
Effect of intraperitoneal injection of immunostimulatory substances on allochthonous gut microbiota of Atlantic salmon (Salmo salar L.) determined using denaturing gradient gel electrophoresis
Version of Record online: 18 MAR 2008
© 2008 The Authors
Volume 39, Issue 6, pages 635–646, April 2008
How to Cite
Liu, Y., Zhou, Z., Yao, B., Shi, P., He, S., Hølvold, L. B. and Ringø, E. (2008), Effect of intraperitoneal injection of immunostimulatory substances on allochthonous gut microbiota of Atlantic salmon (Salmo salar L.) determined using denaturing gradient gel electrophoresis. Aquaculture Research, 39: 635–646. doi: 10.1111/j.1365-2109.2008.01934.x
- Issue online: 18 MAR 2008
- Version of Record online: 18 MAR 2008
- intraperitoneal injection;
- immunostimulatory substances;
- allochthonous gut microbiota;
- Atlantic salmon;
The allochthonous microbiota in the proximal and distal intestine was investigated in three groups of Atlantic salmon (Salmo salar L.) fed a commercial diet and intraperitoneally injected with (a) phosphate-buffered saline (control), (b) lipopolysaccharide (LPS) from the fish pathogenic bacteria, Aeromonas salmonicida ssp. salmonicida, and (c) laminaran [β-(1,3)-d-glucan]. Denaturing gradient gel electrophoresis (DGGE) of the hyper variable V3 region was used to present the microbiota in different experimental groups. Sequencing and phylogenetic analysis of excised DGGE bands suggested that an intraperitoneal injection of LPS from A. salmonicida affects the allochthonous gut bacteria of Atlantic salmon to some extent, as Aeromonas enteropelogenes, Aeromonas veroni, Psychrobacter, Lactobacillus letvazi, Lactobacillus satsumensis, Pantoea, swine manure bacterium and several uncultured bacteria were unique for this group. On the other hand, the bacterial diversity of the allochthonous microbiota did not seem to be affected by injection of β-(1,3)-d-glucan. Sequences of this experimental group were most closely related to A. enteropelogenes, uncultured Escherichia and Lactobacillus aviarius ssp. aviarius.