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Dietary l-lysine requirement of fingerling stinging catfish, Heteropneustes fossilis (Bloch) for optimizing growth, feed conversion, protein and lysine deposition


Correspondence: Mukhtar A. Khan, Fish Nutrition Research Laboratory, Department of Zoology, Aligarh Muslim University, Aligarh 202 002, India. E-mail:


Dietary lysine requirement of fingerling Heteropneustes fossilis (6.96 ± 0.05 g) was quantified by conducting 12-week feeding trial in a flow-through system at 28°C. Casein–gelatin based isonitrogenous (38% CP) and isocaloric (14.7 kJ g−1 DE) amino acid test diets with six levels of dietary lysine (1.5%, 1.75%, 2.0%, 2.25%, 2.5%, 3.0% dry diet) were fed to apparent satiation in triplicates. Broken-line and second-degree polynomial regression analyses at 95% plateau of absolute weight gain (AWG; g fish−1), feed conversion ratio (FCR), protein deposition (PD; g fish−1) and lysine deposition (LD; g fish−1) exhibited lysine requirement between 2.0% to 2.3% of the dry diet, corresponding to 5.3–6.1% protein.