An experiment was carried out to analyse the effects of substituting fish-meal (FM) with soya bean meal and shrimp protein hydrolysate (SPH) on the percent survival, growth rate, feed efficiency and molt frequency of redclaw crayfish hatchlings, Cherax quadricarinatus. The experiment was performed in a closed recirculating-water system over a 56-day period. Five diets were tested: a control diet (SPH0) comprising 100% FM without SPH, and four diets with 2.5%, 5.0%, 10.0% and 15.0% SPH substitution (diets SPH2.5, SPH5, SPH10 and SPH15 respectively). Analysis of water quality indicated that it was adequate to maintain a good growth and survival of hatchlings. The results showed that 100% survival was obtained for hatchlings fed with SPH5, SPH10 and SPH15 diets. Higher mean values of final weight were obtained for the SPH5 diet. The highest weight gain was observed in SPH2.5 and SPH5 diets. Hatchlings showed similar specific growth rates for all diets. The efficiency rate of the SPH15 diet was lower compared to the other diets. Overall, individual feed consumption was higher in hatchlings fed diets of SPH5 and SPH15. It is recommended that 5.0% of SPH could be included in diets of hatchling redclaw crayfish to improve their growth efficiency.