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Lutein enrichment of the rotifer Brachionus sp. using freeze-dried Muriellopsis sp. cells

Authors

  • Stefanie Anne van Bergeijk,

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    • Centro “El Toruño”, IFAPA, Junta de Andalucía, El Puerto de Santa María, Spain
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  • José Antonio Paullada Salmerón,

    1. Centro “El Toruño”, IFAPA, Junta de Andalucía, El Puerto de Santa María, Spain
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  • Ana Isabel López Pérez,

    1. Instituto de Bioquímica Vegetal y Fotosíntesis, Consejo Superior de Investigaciones Científicas-Universidad de Sevilla, Centro de Investigaciones Científicas Isla de Cartuja, Sevilla, Spain
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  • José Moreno,

    1. Instituto de Bioquímica Vegetal y Fotosíntesis, Consejo Superior de Investigaciones Científicas-Universidad de Sevilla, Centro de Investigaciones Científicas Isla de Cartuja, Sevilla, Spain
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  • José Pedro Cañavate,

    1. Centro “El Toruño”, IFAPA, Junta de Andalucía, El Puerto de Santa María, Spain
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  • Mercedes García-González

    1. Instituto de Bioquímica Vegetal y Fotosíntesis, Consejo Superior de Investigaciones Científicas-Universidad de Sevilla, Centro de Investigaciones Científicas Isla de Cartuja, Sevilla, Spain
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Correspondence: S A van Bergeijk, Centro “El Toruño”, IFAPA, Junta de Andalucía, Camino Tiro del Pichón, s/n, 11500 El Puerto de Santa María, Cádiz, Spain. E-mail: stefaniea@juntadeandalucia.es

Abstract

Rotifers, used in aquaculture as feed for early larval stages, are poor in carotenoids compared with copepods, the natural most common prey item of marine fish larvae. A minimal sufficient level of carotenoids in fish larvae may be essential for a correct development. The freshwater microalga Muriellopsis sp. has been identified as a potential source of the carotenoid lutein with possible application in aquaculture. Lutein is a potent antioxidant, with potentially beneficial effects as a carotenoid for fish larvae. This study describes the lutein enrichment of the rotifer Brachionus sp. when incubated with homogeneously dispersed suspensions of freeze-dried, lutein-rich Muriellopsis sp. in seawater. A short enrichment time (2 or 2.5 h) resulted in a higher lutein content of the rotifers than a long enrichment time (13.5 or 24 h). Although the concentration of lutein in enriched rotifers, 0.06–0.16 mg (g dry weight)−1 was low compared with its content in Muriellopsis sp. [about 3 mg (g dry weight)−1], it was in the range reported for natural copepod populations. Therefore, we conclude that freeze-dried biomass of Muriellopsis sp. can be used to enrich rotifers with lutein, providing an adequate level of this carotenoid for marine fish larvae.

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