• H. fossilis ;
  • dietary methionine requirement;
  • growth;
  • biochemical parameters


An 8 weeks feeding trial was conducted to determine the dietary methionine requirement of fingerling Indian catfish, Heteropneustes fossilis (6.08 ± 0.95 cm; 4.33 ± 0.52 g). Six isonitrogenous (40%) and isoenergetic (17.90 kJ g−1 GE) amino acid test diets were formulated with gradation of 0.25 g 100 g−1containing graded levels of L-methionine (0.30, 0.55, 0.80, 1.05, 1.30 and 1.55 g 100 g−1, dry diet) with 0.40 g 100 g−1 constant level of cystine. Twenty fish were stocked in triplicate groups, in 75-L circular trough with continuous flow-through system and fed experimental diets at 4% BW/day twice daily, at 08:00 and 18:00 hours. Maximum live weight gain (296%), best feed conversion ratio (1.56) and protein efficiency ratio (1.60) were occurred at 1.05 g 100 g−1 methionine, beyond which they showed declining tendency. However, quadratic regression analysis of weight gain, feed conversion ratio (FCR), protein efficiency ratio (PER) and body protein deposition (BPD) data indicated requirement for methionine at 1.15, 1.08, 1.06 and 1.05 g 100 g−1 of dry diet respectively. Significantly (< 0.05), higher whole body protein content, minimum moisture and intermediate fat contents were recorded at 1.05 g 100 g−1 dietary methionine level. Ash content remained insignificantly (> 0.05) low among all the treatments, excepting at diet I and diet II. Body protein deposition was also found to be significantly (< 0.05) higher at 1.05 g 100 g−1 methionine level. Best somatic and haematological indices values were also obtained at the requirement level. Based on above results, it is recommended that the diet for young H. fossilis should contain methionine at 1.09 g 100 g−1 dry diet, corresponding to 2.73 g 100 g−1 dietary protein with 0.40 g 100 g1 cystine concentration for optimum growth and efficient feed utilization. Thus, the total sulphur amino acid requirement of H. fossilis would be (1.09 + 0.40) 1.49 g 100 g−1 of dry diet, corresponding to 3.73 g 100 g−1 of dietary protein.