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Ammonia tolerance of Litopenaeus vannamei (Boone) larvae

Authors

  • María de Lourdes Cobo,

    Corresponding author
    1. Escuela Superior Politécnica del Litoral (ESPOL), Centro Nacional de Acuicultura e Investigaciones Marinas (CENAIM), Guayaquil, Ecuador
    • Correspondence: M de Lourdes Cobo, Centro Nacional de Acuicultura e Investigaciones Marinas (CENAIM), Escuela Superior Politécnica del Litoral (ESPOL), Campus Gustavo Galindo Velasco, Km 30.5 vía Perimetral, P.O. Box 09-01-5863, Guayaquil, Ecuador. E-mails: artemia@yahoo.com; mlcobo@cenaim.espol.edu.ec

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  • Stanislaus Sonnenholzner,

    1. Escuela Superior Politécnica del Litoral (ESPOL), Centro Nacional de Acuicultura e Investigaciones Marinas (CENAIM), Guayaquil, Ecuador
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  • Mathieu Wille,

    1. Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Ghent, Belgium
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  • Patrick Sorgeloos

    1. Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Ghent, Belgium
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Abstract

The tolerance of Litopenaeus vannamei larvae to increasing concentrations of total ammonia nitrogen (TAN) using a short-term static renewal method at 26°C, 34 g L−1 salinity and pH 8.5 was assessed. The median lethal concentration (24 h LC50) for TAN in zoea (1-2-3), mysis (1-2-3) and postlarvae 1 were, respectively, 4.2-9.9-16.0; 19.0-17.3-17.5 and 13.2 mg L−1TAN (0.6-1.5-2.4; 2.8-2.5-2.6 and 1.9 mg L−1 NH3-N). The LC50 values obtained in this study suggest that zoeal and post-larval stages are more sensitive to 24 h ammonia exposure than the mysis stage of L. vannamei larvae. On the basis of the ammonia toxicity level (24 h LC50) at zoea 1, we recommend that this level does not exceed 0.42 mg L−1 TAN – equivalent to 0.06 mg L−1 NH3-N – to reduce ammonia toxicity during the rearing of L. vannamei larvae.

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