Effect of a herbal extract containing curcumin and piperine on midazolam, flurbiprofen and paracetamol (acetaminophen) pharmacokinetics in healthy volunteers

Authors

  • Laurie P. Volak,

    1. Program in Pharmacology and Experimental Therapeutics, Sackler School of Graduate Biomedical Sciences, Tufts University, Boston
    Current affiliation:
    1. ArQule, Woburn, Massachusetts, USA
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  • Michael J. Hanley,

    1. Program in Pharmacology and Experimental Therapeutics, Sackler School of Graduate Biomedical Sciences, Tufts University, Boston
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  • Gina Masse,

    1. Department of Molecular Physiology & Pharmacology, Tufts University School of Medicine, Boston, MA, USA
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  • Suwagmani Hazarika,

    1. Department of Molecular Physiology & Pharmacology, Tufts University School of Medicine, Boston, MA, USA
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  • Jerold S. Harmatz,

    1. Department of Molecular Physiology & Pharmacology, Tufts University School of Medicine, Boston, MA, USA
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  • Vladimir Badmaev,

    1. Sabinsa Corporation, East Windsor, NJ, USA
    Current affiliation:
    1. PL Thomas Corporation, Morristown, New Jersey, USA
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  • Muhammed Majeed,

    1. Sabinsa Corporation, East Windsor, NJ, USA
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  • David J. Greenblatt,

    1. Program in Pharmacology and Experimental Therapeutics, Sackler School of Graduate Biomedical Sciences, Tufts University, Boston
    2. Department of Molecular Physiology & Pharmacology, Tufts University School of Medicine, Boston, MA, USA
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  • Michael H. Court

    Corresponding author
    1. Department of Molecular Physiology & Pharmacology, Tufts University School of Medicine, Boston, MA, USA
    • Program in Pharmacology and Experimental Therapeutics, Sackler School of Graduate Biomedical Sciences, Tufts University, Boston
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Correspondence

Dr Michael H. Court, Department of Molecular Physiology and Pharmacology, Tufts University School of Medicine, 136 Harrison Avenue, Boston, MA 02111, USA.

Tel.: +1 617 636 2741

Fax: +1 617 636 6738

E-mail: michael.court@tufts.edu

Abstract

Aims

Turmeric extract derived curcuminoids (curcumin, demethoxycurcumin and bisdemethoxycurcumin) are currently being evaluated for the treatment of cancer and Alzheimer's dementia. Previous in vitro studies indicate that curcuminoids and piperine (a black pepper derivative that enhances curcuminoid bioavailability) could inhibit human CYP3A, CYP2C9, UGT and SULT dependent drug metabolism. The aim of this study was to determine whether a commercially available curcuminoid/piperine extract alters the pharmacokinetic disposition of probe drugs for these enzymes in human volunteers.

Methods

A randomized placebo-controlled six way crossover study was conducted in eight healthy volunteers. A standardized curcuminoid/piperine preparation (4 g curcuminoids plus 24 mg piperine) or matched placebo was given orally four times over 2 days before oral administration of midazolam (CYP3A probe), flurbiprofen (CYP2C9 probe) or paracetamol (acetaminophen) (dual UGT and SULT probe). Plasma and urine concentrations of drugs, metabolites and herbals were measured by HPLC. Subject sedation and electroencephalograph effects were also measured following midazolam dosing.

Results

Compared with placebo, the curcuminoid/piperine treatment produced no meaningful changes in plasma Cmax, AUC, clearance, elimination half-life or metabolite levels of midazolam, flurbiprofen or paracetamol (α = 0.05, paired t-tests). There was also no effect of curcuminoid/piperine treatment on the pharmacodynamics of midazolam. Although curcuminoid and piperine concentrations were readily measured in plasma following glucuronidase/sulfatase treatment, unconjugated concentrations were consistently below the assay thresholds (0.05–0.08 μm and 0.6 μm, respectively).

Conclusion

The results indicate that short term use of this piperine-enhanced curcuminoid preparation is unlikely to result in a clinically significant interaction involving CYP3A, CYP2C9 or the paracetamol conjugation enzymes.

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