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SUMMARY.— The effect of 3 different sampling techniques on forehead surface lipid biochemistry was investigated. Samples obtained by either a sponge method or by ether washings contained significantly more free fatty acid and cholesterol and less triglyceride, wax ester and squalene than did samples collected by absorbent papers.

The reasons for these differences are discussed and it is suggested that more careful attention to detail is necessary in the sampling and handling of surface lipids before biochemical analysis.