Part of this work was presented at the annual meetings of the Society of Investigative Dermatology, Seattle, 1991 and European Society of Dermatological Research, Copenhagen, 1991.
Selective generation of CD8+ T-cell clones from the peripheral blood of patients with cutaneous reactions to beta-lactam antibiotics
Version of Record online: 29 JUL 2006
British Journal of Dermatology
Volume 128, Issue 6, pages 619–626, June 1993
How to Cite
HERTL, M., GEISEL, J., BOECKER, C. and MERK, H.F. (1993), Selective generation of CD8+ T-cell clones from the peripheral blood of patients with cutaneous reactions to beta-lactam antibiotics. British Journal of Dermatology, 128: 619–626. doi: 10.1111/j.1365-2133.1993.tb00255.x
- Issue online: 29 JUL 2006
- Version of Record online: 29 JUL 2006
- Accepted for publication 2 November 1992
The presence, phenotype, and functional characteristics of peripheral blood penicillin-specific T lymphocytes in individuals with cutaneous allergic reactions to penicillin were investigated using in vitro long-term culture techniques. Peripheral blood mononuclear cells from two penicillin-allergic patients were stimulated in vitro with penicillin, and T-cell blasts were clonally expanded by limiting dilution. Seven T-cell clones were derived, all of which were CD3+ CD4− CD8+ HLA-DR+, and produced IL-2 and IFN-γ upon stimulation. T-cell proliferation required the presence of antigen and autologous, but not allogeneic, antigen-presenting cells. In addition to the parent compound, the T-cell clones also developed a proliferative response to penicilloyl, the major metabolite of penicillin. The cloned T-cell lines were found to exhibit marked suppressor activity for Con A mitogenesis. The observed suppressor activity required cell-to-cell contact, as supernatants from these T-cell clones had no comparable inhibitory effect. These findings indicate that there is a predominance of penicillin-specific CD8+ T cells in the peripheral blood of individuals sensitized to beta-lactam antibiotics.