Association between melanoma and dermal mast cell prevalence in sun-unexposed skin

Authors

  • M.A. Grimbaldeston,

    1. Department of Microbiology and Infectious Diseases, School of Medicine and Flinders Medical Research Institute, Flinders University, GPO Box 2100, Adelaide, South Australia 5001, Australia
      *Adelaide Melanoma Unit, Department of Surgery, University of Adelaide, Adelaide, South Australia, Australia
      †Department of Dermatology, Flinders Medical Centre, Adelaide, South Australia, Australia
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  • A.L. Pearce,

    1. Department of Microbiology and Infectious Diseases, School of Medicine and Flinders Medical Research Institute, Flinders University, GPO Box 2100, Adelaide, South Australia 5001, Australia
      *Adelaide Melanoma Unit, Department of Surgery, University of Adelaide, Adelaide, South Australia, Australia
      †Department of Dermatology, Flinders Medical Centre, Adelaide, South Australia, Australia
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  • B.O. Robertson,

    1. Department of Microbiology and Infectious Diseases, School of Medicine and Flinders Medical Research Institute, Flinders University, GPO Box 2100, Adelaide, South Australia 5001, Australia
      *Adelaide Melanoma Unit, Department of Surgery, University of Adelaide, Adelaide, South Australia, Australia
      †Department of Dermatology, Flinders Medical Centre, Adelaide, South Australia, Australia
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  • B.J. Coventry,

    1. Department of Microbiology and Infectious Diseases, School of Medicine and Flinders Medical Research Institute, Flinders University, GPO Box 2100, Adelaide, South Australia 5001, Australia
      *Adelaide Melanoma Unit, Department of Surgery, University of Adelaide, Adelaide, South Australia, Australia
      †Department of Dermatology, Flinders Medical Centre, Adelaide, South Australia, Australia
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  • G. Marshman,

    1. Department of Microbiology and Infectious Diseases, School of Medicine and Flinders Medical Research Institute, Flinders University, GPO Box 2100, Adelaide, South Australia 5001, Australia
      *Adelaide Melanoma Unit, Department of Surgery, University of Adelaide, Adelaide, South Australia, Australia
      †Department of Dermatology, Flinders Medical Centre, Adelaide, South Australia, Australia
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  • J.J. Finlay-Jones,

    1. Department of Microbiology and Infectious Diseases, School of Medicine and Flinders Medical Research Institute, Flinders University, GPO Box 2100, Adelaide, South Australia 5001, Australia
      *Adelaide Melanoma Unit, Department of Surgery, University of Adelaide, Adelaide, South Australia, Australia
      †Department of Dermatology, Flinders Medical Centre, Adelaide, South Australia, Australia
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  • P.H. Hart

    1. Department of Microbiology and Infectious Diseases, School of Medicine and Flinders Medical Research Institute, Flinders University, GPO Box 2100, Adelaide, South Australia 5001, Australia
      *Adelaide Melanoma Unit, Department of Surgery, University of Adelaide, Adelaide, South Australia, Australia
      †Department of Dermatology, Flinders Medical Centre, Adelaide, South Australia, Australia
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  • M.A.G. and A.L.P. contributed equally to this manuscript.

Prue H. Hart, Telethon Institute for Child Health Research, PO Box 855, West Perth, Western Australia 6872, Australia.
E-mail: prueh@ichr.uwa.edu.au

Summary

Background  Both exposure to intermittent intense sunlight during childhood and ultraviolet (UV) radiation-induced immunomodulation have been directly associated with melanoma development. In mice, the prevalence of dermal mast cells determines susceptibility to UVB-induced systemic suppression of contact hypersensitivity responses and thus may affect immunological responses to melanoma antigens.

Objectives  To determine the relevance of murine studies of dermal mast cell prevalence to human melanoma pathogenesis.

Methods  The prevalence of mast cells was examined in sun-unexposed buttock skin of 45 melanoma patients and 68 control volunteers who had no history of skin cancer development. Buttock skin was studied because mast cell prevalence is stable with ageing and the confounding effects of environmental UV exposure are minimized.

Results  Using tissue immunostaining, the buttock skin from melanoma patients had a significantly higher dermal mast cell prevalence (mean ± SEM 38 ± 2 mast cells mm−2) than controls (32 ± 2 mast cells mm−2) (P = 0·02). Analysis by binary logistic regression showed that the association between mast cell prevalence and melanoma outcome was not significantly altered by skin phototype.

Conclusions  The immunomodulatory effects of mast cell products in UV-irradiated skin may contribute significantly to the initiation and development of human cutaneous malignant melanoma.

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