Protection from photodamage by topical application of caffeine after ultraviolet irradiation

Authors

  • S-W. Koo,

    1. Cutaneous Biology Research Center, Massachusetts General Hospital, Harvard Medical School, 149 13th St, Charlestown, MA 02129, U.S.A.
      *Current address: Department of Dermatology, Ehime University School of Medicine, Toon, Ehime 791-0295, Japan
      †Current address: Department of Medicine, Division of Dermatology, University of Washington, 815 Mercer St, Seattle, WA 98109, U.S.A.
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  • S. Hirakawa,

    1. Cutaneous Biology Research Center, Massachusetts General Hospital, Harvard Medical School, 149 13th St, Charlestown, MA 02129, U.S.A.
      *Current address: Department of Dermatology, Ehime University School of Medicine, Toon, Ehime 791-0295, Japan
      †Current address: Department of Medicine, Division of Dermatology, University of Washington, 815 Mercer St, Seattle, WA 98109, U.S.A.
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  • S. Fujii,

    1. Cutaneous Biology Research Center, Massachusetts General Hospital, Harvard Medical School, 149 13th St, Charlestown, MA 02129, U.S.A.
      *Current address: Department of Dermatology, Ehime University School of Medicine, Toon, Ehime 791-0295, Japan
      †Current address: Department of Medicine, Division of Dermatology, University of Washington, 815 Mercer St, Seattle, WA 98109, U.S.A.
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  • M. Kawasumi,

    1. Cutaneous Biology Research Center, Massachusetts General Hospital, Harvard Medical School, 149 13th St, Charlestown, MA 02129, U.S.A.
      *Current address: Department of Dermatology, Ehime University School of Medicine, Toon, Ehime 791-0295, Japan
      †Current address: Department of Medicine, Division of Dermatology, University of Washington, 815 Mercer St, Seattle, WA 98109, U.S.A.
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  • P. Nghiem

    1. Cutaneous Biology Research Center, Massachusetts General Hospital, Harvard Medical School, 149 13th St, Charlestown, MA 02129, U.S.A.
      *Current address: Department of Dermatology, Ehime University School of Medicine, Toon, Ehime 791-0295, Japan
      †Current address: Department of Medicine, Division of Dermatology, University of Washington, 815 Mercer St, Seattle, WA 98109, U.S.A.
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  • Conflicts of interest None declared.

P. Nghiem.
E-mail: pnghiem@u.washington.edu

Summary

Background  Characterization of mechanisms that can reverse residual damage from prior skin exposure to ultraviolet (UV) would be of considerable biological and therapeutic interest. Topical caffeine application to mouse skin that had previously been treated with UV has been shown to inhibit the subsequent development of squamous cell carcinomas.

Objectives  We used an established mouse photodamage model to investigate other possible effects of topical caffeine application after UV.

Methods  SKH-1 hairless mice were treated with ultraviolet B (UVB) followed immediately by topical application of caffeine or vehicle three times weekly for 11 weeks.

Results  Caffeine applied topically after UV treatment resulted in a significant decrease in UV-induced skin roughness/transverse rhytides as assessed by treatment-blinded examiners. Histologically, topical caffeine application after a single dose of UVB more than doubled the number of apoptotic keratinocytes as evaluated by sunburn cell formation, caspase 3 cleavage and terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick-end labelling (TUNEL) staining. A trend towards decreased solar elastosis was noted in the caffeine-treated group although this was not statistically significant. Other histological parameters including epidermal hyperplasia, solar elastosis and angiogenesis were increased in mice treated with UV but topical application of caffeine did not alter these particular UV effects.

Conclusions  These findings support the concept that topical application of caffeine to mouse skin after UV irradiation promotes the deletion of DNA-damaged keratinocytes and may partially diminish photodamage as well as photocarcinogenesis.

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