Conflicts of interest None declared.
Biphasic expression of stromal cell-derived factor-1 during human wound healing
Article first published online: 18 OCT 2007
British Journal of Dermatology
Volume 157, Issue 6, pages 1148–1154, December 2007
How to Cite
Toksoy, A., Müller, V., Gillitzer, R. and Goebeler, M. (2007), Biphasic expression of stromal cell-derived factor-1 during human wound healing. British Journal of Dermatology, 157: 1148–1154. doi: 10.1111/j.1365-2133.2007.08240.x
- Issue published online: 18 OCT 2007
- Article first published online: 18 OCT 2007
- Accepted for publication 11 July 2007
- endothelial cells;
- stromal cell-derived factor-1;
- wound healing
Background Chemokines tightly regulate the spatial and temporal infiltration of invading leucocyte subsets during wound healing. Stromal cell-derived factor-1 (SDF-1/CXCL12) is a homeostatic chemokine with multiple functions; its role during cutaneous wound healing, however, needs to be explored.
Objectives To elucidate expression of the multifunctional CXC chemokine SDF-1/CXCL12 during human wound healing.
Methods Skin biopsies were obtained from 14 volunteers between 1 and 21 days after incisional wounding and processed for in situ hybridization and immunohistochemistry.
Results We analysed the spatial and temporal distribution of SDF-1/CXCL12 after artificial wounding and detected a complete downregulation at both the mRNA and the protein level within the fibrous stroma that replaces the initial wound defect. However, increased levels of SDF-1/CXCL12 were observed at the wound margins. Focusing on mediators regulating SDF-1/CXCL12 expression in vitro we realized that both tumour necrosis factor-α and interferon-γ downregulated its expression in human dermal microvascular endothelial cells and fibroblasts.
Conclusions Our data suggest that SDF-1/CXCL12 is tightly regulated during wound repair. Increased expression at the wound margin may contribute to the accumulation of endothelial progenitor cells, thus accelerating neovascularization.