Conflicts of interest None declared.
Abnormal AP-1 protein expression in primary cutaneous B-cell lymphomas
Article first published online: 2 MAY 2008
© 2008 The Authors. Journal Compilation © 2008 British Association of Dermatologists
British Journal of Dermatology
Volume 159, Issue 1, pages 145–151, July 2008
How to Cite
Mao, X. and Orchard, G. (2008), Abnormal AP-1 protein expression in primary cutaneous B-cell lymphomas. British Journal of Dermatology, 159: 145–151. doi: 10.1111/j.1365-2133.2008.08579.x
- Issue published online: 2 MAY 2008
- Article first published online: 2 MAY 2008
- Accepted for publication 11 February 2008
- AP-1 transcription factors;
- primary cutaneous B-cell lymphoma;
- primary cutaneous follicle centre lymphoma;
- primary cutaneous large B-cell lymphoma;
- leg type;
- primary cutaneous marginal zone B-cell lymphoma
Background Primary cutaneous B-cell lymphoma (PCBCL) consists mainly of primary cutaneous marginal zone B-cell lymphoma (PCMZL), primary cutaneous follicle centre lymphoma (PCFCL) and primary cutaneous large B-cell lymphoma, leg type (PCLBCL-LT). The activator protein 1 (AP-1) transcription factor includes JUN, FOS and other family members.
Objectives To assess the expression pattern of AP-1 transcription factors in PCBCL.
Methods We analysed paraffin tissue sections from nine cases of PCMZL, seven PCFCL, six PCLBCL-LT and two unspecified PCBCL cases by using immunohistochemistry with antibodies against c-JUN, JUNB, JUND, c-FOS, RAF1, αPAK, CD30 and CCND1.
Results A positive staining for JUND (++) was observed in six cases of PCFCL (86%), five PCLBCL-LT (83%) and five PCMZL (56%). Positive CCND1 protein expression was present in four cases of PCLBCL-LT (67%), four PCFCL (57%) and four PCMZL (44%), and the two unspecified PCBCL cases. Expression of αPAK protein was seen in three cases of PCLBCL-LT (50%), two PCMZL (22%) and one PCFCL. However, c-JUN, c-FOS and RAF1 protein were rarely expressed in the PCBCL cases analysed; JUNB and CD30 protein expression was absent in these cases.
Conclusions These findings suggest that the presence of abnormal AP-1 protein expression is associated with upregulation of JUND, CCND1 and αPAK and downregulation of JUNB in PCBCL.