Relevance of compartmentalization of T-cell subsets for clinical improvement in psoriasis: effect of immune-targeted antipsoriatic therapies

Authors


  • Conflicts of interest
    Prof. Dr P.C.M. van de Kerkhof has received funds for research, fees for educational events and fees for consultancy from the following companies: Schering Plough, Cellgene, Centocor, Almirall, UCB, Wyeth, Pfizer, Sofinova, Abbott, Actelion, Galderma, Novartis, Janssen Cilag, Leo Pharma, Merck Serono, Philips Lighting. Dr E.M.G.J. de Jong serves as a consultant for Biogen, Serono, Wyeth, and Abbott. Dr E.M.G.J. de Jong receives research grants from Schering Plough, Abbott, Merck Serono, Wyeth, and Centocor.

Rosanne G. van Lingen.
E-mail: R.vanlingen@derma.umcn.nl

Summary

Background  Therapies targeting the T cell-mediated pathology of psoriasis have been found to achieve remarkable clinical improvement and have confirmed the crucial role of the immune system either in peripheral blood (PB) or in skin. No analyses of T-cell counts in both compartments have been conducted in order to confirm or refute the hypothesized shifts between them.

Objectives  To gain more insight in the dynamics of compartmentalization of T cells between PB and lesional skin of patients with psoriasis, in response to immune-targeted antipsoriatic therapies.

Methods  Eighteen patients with psoriasis received either efalizumab (= 9) or etanercept (= 9) for 12 weeks. Biopsies were taken for immunohistochemical analysis of T-cell subsets and simultaneously T-cell subsets were isolated from PB specimens by flow cytometry.

Results  The Psoriasis Area and Severity Index declined significantly after 12 weeks of etanercept, but not for efalizumab. After treatment with efalizumab, a significantly decreased number of all T-cell subsets was found in the dermis. In the epidermis, CD4+, CD8+, CD25+, CD45RO+ and CD161+ T-cell subsets were significantly decreased. With respect to etanercept, few significant changes in T-cell subsets were found. The percentage of lymphocytes in PB was significantly elevated after efalizumab treatment regardless of responder status.

Conclusions  Treatment with efalizumab establishes successful recompartmentalization of T-cell subsets with modest clinical efficacy after 12 weeks, whereas in etanercept-treated patients, a significant clinical response is no guarantee for significant changes in T-cell subsets in the different compartments. Reductions in T-cell subsets cannot be used as predictive markers for the clinical response to therapy. Interference with the studied T-cell populations in its own right seems not to be responsible for the clinical efficacy of efalizumab and etanercept.

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