Conflicts of interest None declared.
Psoralen plus ultraviolet A ± interferon-α treatment resistance in mycosis fungoides: the role of tumour microenvironment, nuclear transcription factor-κB and T-cell receptor pathways
Article first published online: 16 OCT 2008
© 2008 The Authors. Journal Compilation © 2008 British Association of Dermatologists
British Journal of Dermatology
Volume 160, Issue 1, pages 92–102, January 2009
How to Cite
Wozniak, M.B., Tracey, L., Ortiz-Romero, P.L., Montes, S., Alvarez, M., Fraga, J., Fernández Herrera, J., Vidal, S., Rodriguez-Peralto, J.L., Piris, M.Á. and Villuendas (deceased), R. (2009), Psoralen plus ultraviolet A ± interferon-α treatment resistance in mycosis fungoides: the role of tumour microenvironment, nuclear transcription factor-κB and T-cell receptor pathways. British Journal of Dermatology, 160: 92–102. doi: 10.1111/j.1365-2133.2008.08886.x
M.B.W. and L.T. contributed equally to this work.
- Issue published online: 15 DEC 2008
- Article first published online: 16 OCT 2008
- Accepted for publication 19 August 2008
- interferon alpha;
- microarray analysis;
- mycosis fungoides;
- psoralen plus ultraviolet A
Background Interferon (IFN)-α is widely used in the treatment of mycosis fungoides (MF) and when used in combination with photochemotherapy (psoralen plus ultraviolet A, PUVA) both improved response and duration of complete remission have been reported. However, in spite of encouraging results of the initial studies, currently there is no information available on specific prognostic factors enabling prediction of patients’ resistance to PUVA ± IFN-α treatment.
Objectives To identify factors responsible for resistance to PUVA ± IFN-α treatment in MF patients.
Patients/methods The gene expression profiling of pretreatment samples from 29 patients diagnosed as IA, IB or IIA stage of MF enrolled in a randomized PUVA vs. PUVA + IFN-α clinical trial was analysed using cDNA microarrays. A Cox model (SAM) and gene set enrichment analysis (GSEA) were used for identification of genes and biologically significant pathways related to resistance to treatment.
Results Genes involved in NF-κB signalling, T-cell receptor (TCR) signalling, cytokine signalling and proliferation were differentially expressed between responders and nonresponders. Interestingly, expression of markers representative of those pathways was found not only in the tumoral cells, but also in specific subpopulations of macrophages, dendritic cells and other non-neoplastic cell types constituting the tumour microenvironment, likely involved in the promotion of survival and proliferation of cutaneous T-cell lymphoma.
Conclusions Gene expression changes in both the tumour and the tumour microenvironment are an important determinant of treatment outcome in early-stage MF patients. Some proinflammatory factors such as NF-κB, inflammatory cytokines and their receptors in addition to TCR-associated molecules could be promising targets for MF treatment.