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Figure S1. (a) An example of quantitative histomorphometry measuring of MIF intensity staining in epithelium and in dermis by using NIH ImageJ software. (b, c) LL-37 immunoreactivity is upregulated in the epidermis (ep) and dermis (der) of HS skin with inflammation compared with control. (d, e) Protein expression of α-MSH in HS skin with inflammation expression of α-MSH in the epidermis (ep) compared with control. (f, g) Lysozyme immunoreactivity is decreased in the epidermis (ep) in scarring HS skin compared with control. (h, i) hBD3 expression is increased in the epidermis of HS skin with inflammation (ep) and in the isthmus and the proximal ORS compared with control. Haematoxylin and eosin, original magnification: (a, b, d, f, h) × 400. *P ≤ 0·05, **P ≤ 0·01, ***P ≤ 0·001, ± SEM, P-value was calculated by Mann–Whitney U-test. der, dermis; ep, epidermis; hBD3, human β-defensin 3; HS, hidradenitis suppurativa; IRS, inner root sheath; MIF, macrophage migration inhibitory factor; MSH, melanocyte stimulating hormone; ORS, outer root sheath.

Figure S2. (a, b) Expression of psoriasin is increased in the epidermis (ep) and infundibulum or distal (ORS) part of the ORS in HS skin with inflammation compared with control. In addition, the HS specimen with inflammation shows upregulated psoriasin immunoreactivity in the proximal ORS (ORS). (c, d) Protein expression of IL-8 in HS skin with inflammation expression of IL-8 in the epidermis (ep) compared with control. (e, f) Expression of TNF-α (a–f) is increased in HS skin with inflammation or scarring in the epidermis (ep) and in the dermis compared with control skin. In addition, HS samples with inflammation or scarring show less TNF-α staining intensity in the isthmus and proximal part of the ORS. (g, h) MIF expression significant increased in HS skin with scarring compared with control. der, dermis; ep, epidermis; HS, hidradenitis suppurativa; IL, interleukin; IRS, inner root sheath; MIF, macrophage migration inhibitory factor; MSH, melanocyte stimulating hormone; ORS, outer root sheath; TNF, tumour necrosis factor.

FilenameFormatSizeDescription
BJD_10765_sm_FigS1.JPG121KSupporting info item
BJD_10765_sm_FigS2.JPG129KSupporting info item

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