Current affiliation: Department of Dermatology, Boston University School of Medicine, 609 Albany Street, Boston, MA, 02118, U.S.A.
Immunohistological pointers to a possible role for excessive cathelicidin (LL-37) expression by apocrine sweat glands in the pathogenesis of hidradenitis suppurativa/acne inversa
Article first published online: 4 APR 2012
© 2011 The Authors. BJD © 2011 British Association of Dermatologists
British Journal of Dermatology
Volume 166, Issue 5, pages 1023–1034, May 2012
How to Cite
Emelianov, V.U., Bechara, F.G., Gläser, R., Langan, E.A., Taungjaruwinai, W.M., Schröder, J.M., Meyer, K.C. and Paus, R. (2012), Immunohistological pointers to a possible role for excessive cathelicidin (LL-37) expression by apocrine sweat glands in the pathogenesis of hidradenitis suppurativa/acne inversa. British Journal of Dermatology, 166: 1023–1034. doi: 10.1111/j.1365-2133.2011.10765.x
Funding sources This study was supported in part by DFG Centre of Excellence, ‘Inflammation at Interfaces’. E.A.L. is supported by a Medical Research Council Clinical Research Training Fellowship.
Conflicts of interest None declared.
F.G.B, R.G., E.A.L. and W.M.T. contributed equally to this work.
- Issue published online: 23 APR 2012
- Article first published online: 4 APR 2012
- Accepted manuscript online: 2 DEC 2011 09:43AM EST
- Accepted for publication 27 November 2011
Figure S1. (a) An example of quantitative histomorphometry measuring of MIF intensity staining in epithelium and in dermis by using NIH ImageJ software. (b, c) LL-37 immunoreactivity is upregulated in the epidermis (ep) and dermis (der) of HS skin with inflammation compared with control. (d, e) Protein expression of α-MSH in HS skin with inflammation expression of α-MSH in the epidermis (ep) compared with control. (f, g) Lysozyme immunoreactivity is decreased in the epidermis (ep) in scarring HS skin compared with control. (h, i) hBD3 expression is increased in the epidermis of HS skin with inflammation (ep) and in the isthmus and the proximal ORS compared with control. Haematoxylin and eosin, original magnification: (a, b, d, f, h) × 400. *P ≤ 0·05, **P ≤ 0·01, ***P ≤ 0·001, ± SEM, P-value was calculated by Mann–Whitney U-test. der, dermis; ep, epidermis; hBD3, human β-defensin 3; HS, hidradenitis suppurativa; IRS, inner root sheath; MIF, macrophage migration inhibitory factor; MSH, melanocyte stimulating hormone; ORS, outer root sheath.
Figure S2. (a, b) Expression of psoriasin is increased in the epidermis (ep) and infundibulum or distal (ORS) part of the ORS in HS skin with inflammation compared with control. In addition, the HS specimen with inflammation shows upregulated psoriasin immunoreactivity in the proximal ORS (ORS). (c, d) Protein expression of IL-8 in HS skin with inflammation expression of IL-8 in the epidermis (ep) compared with control. (e, f) Expression of TNF-α (a–f) is increased in HS skin with inflammation or scarring in the epidermis (ep) and in the dermis compared with control skin. In addition, HS samples with inflammation or scarring show less TNF-α staining intensity in the isthmus and proximal part of the ORS. (g, h) MIF expression significant increased in HS skin with scarring compared with control. der, dermis; ep, epidermis; HS, hidradenitis suppurativa; IL, interleukin; IRS, inner root sheath; MIF, macrophage migration inhibitory factor; MSH, melanocyte stimulating hormone; ORS, outer root sheath; TNF, tumour necrosis factor.
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