Methods S1. Sample collection and preparation; genotyping for exclusion of candidate genes and loci; genome-wide linkage analysis; mutation screening of candidate genes by Sanger sequencing; cloning of FZD6 and protein analyses; immunohistochemistry.

Figure S1. Hair whorls of individuals affected with nail dysplasia.

Figure S2. Presentation of the identified candidate region.

Figure S3. Haplotype analysis of family A and B using microsatellites covering the region around the FZD6 gene.

Figure S4. Heterologous expression of FZD6 mutant and wild-type proteins.

Table S1. Primers for cloning and sequencing of FZD6 wild-type and mutant constructs.

Table S2. Expression of frizzled proteins in the hair follicle and the nail.

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