Funding sources Financial support to O.A.A. was provided through regional agreement between Umeå University and Västerbotten County Council on cooperation in the field of Medicine, Odontology and Health. A.McD. was funded by a grant awarded to S.P. from the R & D Office of Health and Social Care Northern Ireland.
CLINICAL AND LABORATORY INVESTIGATIONS
An increased incidence of Propionibacterium acnes biofilms in acne vulgaris: a case–control study
Article first published online: 1 JUN 2012
© 2012 The Authors. BJD © 2012 British Association of Dermatologists
British Journal of Dermatology
Volume 167, Issue 1, pages 50–58, July 2012
How to Cite
Jahns, A.C., Lundskog, B., Ganceviciene, R., Palmer, R.H., Golovleva, I., Zouboulis, C.C., McDowell, A., Patrick, S. and Alexeyev, O.A. (2012), An increased incidence of Propionibacterium acnes biofilms in acne vulgaris: a case–control study. British Journal of Dermatology, 167: 50–58. doi: 10.1111/j.1365-2133.2012.10897.x
Conflicts of interest None declared.
- Issue published online: 28 JUN 2012
- Article first published online: 1 JUN 2012
- Accepted manuscript online: 22 FEB 2012 01:57PM EST
- Accepted for publication 10 February 2012
Summary Background Acne vulgaris is a disorder of the sebaceous follicles. Propionibacterium acnes can be involved in inflammatory acne.
Objectives This case–control study aimed at investigating the occurrence and localization of P. acnes in facial biopsies in acne and to characterize the P. acnes phylotype in skin compartments.
Methods Specific monoclonal and polyclonal antibodies were applied to skin biopsies of 38 patients with acne and matching controls to localize and characterize P. acnes and to determine expression of co-haemolysin CAMP factor, a putative virulence determinant.
Results Follicular P. acnes was demonstrated in 18 (47%) samples from patients with acne and eight (21%) control samples [odds ratio (OR) 3·37, 95% confidence interval (CI) 1·23–9·23; P = 0·017]. In 14 (37%) samples from patients with acne, P. acnes was visualized in large macrocolonies/biofilms in sebaceous follicles compared with only five (13%) control samples (OR 3·85, 95% CI 1·22–12·14; P = 0·021). Macrocolonies/biofilms consisting of mixed P. acnes phylotypes expressing CAMP1 were detected in both case and control samples. Only four samples tested positive for the presence of Staphylococcus spp. and fungi were not observed.
Conclusions We have for the first time visualized different P. acnes phylotypes in macrocolonies/biofilms in sebaceous follicles of skin biopsies. Our results support the hypothesis that P. acnes can play a role in the pathogenesis of acne as acne samples showed a higher prevalence of follicular P. acnes colonization, both in terms of follicles containing P. acnes and the greater numbers of bacteria in macrocolonies/biofilms than in control samples.