Some Immunological Investigations on Antithrombin III ‘Budapest’

Authors


Dr Janet L. Lane, Department of Clinical Haematoloyg and Oncology, Royal Children's Hospital, Parkville, Melbourne, Australia 3052.

Abstract

Summary. Immunoelectrophoretic studies were carried out on plasma from a patient with an abnormal antithrombin III molecule (antithrombin III ‘Budapest’). One-dimensional immunoelectrophoresis using a commercial antibody to antithrombin III (antibody to α2,-AtIII) showed two precipitin peaks of α2-AtIII antigen. Two-dimensional immunoelectrophoresis showed two precipitin peaks, one with normal electrophoretic mobility, the other with increased electrophoretic mobility. It was shown that α2-AtIII antigen with normal mobility was identical to normal α2-AtIII, and that the α2-AtIII antigen with increased electrophoretic mobility was antigenically deficient, and appeared to be present in higher concentration than the normal α2-AtIII antigen. Although one-dimensional immunoelectrophoresis on plasma from the patient's son showed only one peak, two-dimensional immunoelectrophoresis revealed that the son also had two populations of α2-AtIII, one with normal mobility, the other with increased mobility. However, the α2-AtIII antigen with normal mobility appeared to be present in higher concentration than the α2-AtIII antigen with increased mobility. One- and two-dimensional immunoelectrophoresis using a different commercial antibody to α2-AtIII showed only one precipitin peak using the patient's plasma. The precipitin peak observed following two-dimensional immunoelectrophoresis was asymmetric and showed increased mobility.

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