Present address: Walter and Eliza Hall Institute of Medical Research, Post Office, Royal Melbourne Hospital, Victoria 3050, Australia.
Differentiation antigens on mouse eosinophils and neutrophils identified by monoclonal antibodies
Version of Record online: 7 JUL 2008
British Journal of Haematology
Volume 57, Issue 3, pages 489–494, July 1984
How to Cite
Lopez, A. F., Strath, M. and Sanderson, C. J. (1984), Differentiation antigens on mouse eosinophils and neutrophils identified by monoclonal antibodies. British Journal of Haematology, 57: 489–494. doi: 10.1111/j.1365-2141.1984.tb02923.x
- Issue online: 7 JUL 2008
- Version of Record online: 7 JUL 2008
- Received 21 July 1983; accepted for publication 9 November 1983
Summary. A panel of hybridomas secreting monoclonal antibodies (mAb) reacting with antigens on mouse eosinophils and neutrophils has been selected. It is shown that four mAb bind preferentially to eosinophils, recognizing antigens expressed between 5 and 25 times more densely on these cells than on neutrophils. One mAb reacts preferentially with neutrophils, binding an antigen about 12 times more common on these cells than on eosinophils. Four mAb are shown to react with both eosinophils and neutrophils, and also macrophages. These mAb confirm the presence of different differentiation antigens on both eosinophils and neutrophils, and show that the myeloid series express antigens not expressed on lymphocytes.