Summary. The platelet population in man and rat can be divided into two classes of about equal size based on the presence/absence of a p-nitrophenylphosphatase, which probably is a phosphotyrosine phosphatase (PTPase). Phosphorylation of tyrosines on several platelet proteins is implicated in platelet activation, and I carried out in vitro and in vivo experiments on rats to determine whether PTPase positive and negative platelets differed in their reaction time. I used adhesion to collagen in vitro and in vivo (longitudinal slits in aorta and vena portae) and platelet aggregates in clots formed in vivo. I present evidence that PTPase negative platelets react the fastest, most conspicuously seen in the arterial bleeding under high flow conditions, where the first platelets to respond and adhere are predominantly PTPase negative.