The views of these authors represent scientific opinion and should not be construed as FDA policy.
Elevated circulating endothelial membrane microparticles in paroxysmal nocturnal haemoglobinuria
Article first published online: 5 MAY 2004
British Journal of Haematology
Volume 125, Issue 6, pages 804–813, June 2004
How to Cite
Simak, J., Holada, K., Risitano, A. M., Zivny, J. H., Young, N. S. and Vostal, J. G. (2004), Elevated circulating endothelial membrane microparticles in paroxysmal nocturnal haemoglobinuria. British Journal of Haematology, 125: 804–813. doi: 10.1111/j.1365-2141.2004.04974.x
- Issue published online: 5 MAY 2004
- Article first published online: 5 MAY 2004
- Received 4 February 2004; accepted for publication 10 March 2004
- adhesion molecules;
- endothelial cells;
- flow cytometry;
- paroxysmal nocturnal haemoglobinuria;
- sickle cell disease
We analysed endothelial cell membrane microparticles (ECMP) in the peripheral blood of patients with paroxysmal nocturnal haemoglobinuria (PNH) (n = 9), aplastic anaemia (AA) (n = 10), sickle cell disease (SCD) (n = 8), and healthy donors (HD) (n = 11). There was no clinically manifested thrombosis in the PNH or AA group, except one cured thrombophlebitis (PNH), while all SCD patients had a history of vaso-occlusive crises. We used three-colour flow cytometry with blood cell-specific antibodies and antibodies to endothelial antigens CD105 and CD144. Phosphatidylserine-positive microparticles were detected using the annexin V-binding (AVB) assay. The population of CD105+AVB+ ECMP was significantly (P < 0·05) higher in SCD (median: 0·568 × 109/l; 25–75th percentile range: 0·351–0·976 × 109/l) and PNH (0·401 × 109/l ; 0·19–0·441 × 109/l) patients when compared with AA (0·122 × 109/l; 0·061–0·172 × 109/l) or HD (0·180 × 109/l; 0·137–0·217 × 109/l) group. Even more pronounced differences were observed in ECMP exhibiting a marker of inflammatory stimulation CD54 (CD105+CD54+). Similarly, ECMP that exhibited endothelial specific and proteolysis-sensitive antigen CD144 were increased in SCD and PNH, but not in AA. Elevated CD54+ ECMP may reflect the inflammatory status of endothelial cells in SCD and PNH, while CD144+ ECMP could indicate continuous endothelial stimulation and/or injury. Analysis of circulating ECMP appears promising to provide useful information on the status of the vascular endothelium in PNH and SCD.