Resolution of ambiguous low-level positive quantitative polymerase chain reaction results in TEL-AML1 positive ALL using a post-PCR fluorescent oligoligation method*

Authors

  • I-Ming Chen,

    1. Department of Pathology, University of New Mexico HSC and Cancer Research and Treatment Center, Albuquerque, NM USA
    2. Children's Oncology Group Operations Center, Arcadia, CA, USA
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  • Artemis Chakerian,

    1. Department of Pathology, University of New Mexico HSC and Cancer Research and Treatment Center, Albuquerque, NM USA
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  • Meenakshi Devidas,

    1. Children's Oncology Group Operations Center, Arcadia, CA, USA
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  • Michael J. Borowitz,

    1. Children's Oncology Group Operations Center, Arcadia, CA, USA
    2. Johns Hopkins Medical Institutions, Baltimore, MD, USA
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  • Stephen P. Hunger,

    1. Children's Oncology Group Operations Center, Arcadia, CA, USA
    2. Department of Pediatric Oncology, University of Florida, Gainesville, FL, USA
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  • Cheryl L. Willman,

    1. Department of Pathology, University of New Mexico HSC and Cancer Research and Treatment Center, Albuquerque, NM USA
    2. Children's Oncology Group Operations Center, Arcadia, CA, USA
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  • David S. Viswanatha

    1. Children's Oncology Group Operations Center, Arcadia, CA, USA
    2. Mayo Clinic, Division of Hematopathology, Rochester, MN, USA
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  • *

    This work was supported by a Children's Oncology Group Translational Research Award U10 CA98543-01 to DSV.

I-Ming Chen, Children's Oncology Group, PO Box 60012, Arcadia, CA, USA. E-mail:ichen@salud.unm.edu.

Summary

The interpretation of low-level or non-reproducible amplification results in clinical quantitative polymerase chain reaction (Q-PCR) assays can be difficult to definitively resolve. Concerning minimal residual disease detection in leukaemia, indeterminate low-level results might create prognostic or therapeutic dilemmas. We evaluated low-level, ambiguous Q-PCR results in a study of paired diagnostic and end-induction (day 29) TEL-AML1 positive acute lymphoblastic leukaemia samples utilising a novel fluorescent primer ligation detection assay. The data presented here indicate that a significant number of low-level apparent Q-PCR positive results may be spurious or non-specific in nature, requiring additional technical manoeuvres for confirmation of true positive cases.

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