Expression and production of aberrant PAX5 with deletion of exon 8 in B-lineage acute lymphoblastic leukaemia of children

Authors


Dr E. Ishii, Department of Paediatrics, Saga University, 5-1-1 Nabeshima, Saga 849-8501, Japan.
E-mail: ishiei@med.saga-u.ac.jp

Summary

We investigated PAX5 expression in childhood B-lineage acute lymphoblastic leukaemia (ALL). Seven of 21 children with B-lineage ALL had multiple PAX5 variants, while 14 children and healthy controls showed full-length (FL) and one variant PAX5. By Western blotting, healthy controls displayed Pax5-FL, while one short Pax5, derived from the deletion of exon 8 (Pax5-ΔE8) was produced in 90% of ALL samples, as well as in ALL cell lines. PAX5-ΔE8 lacked more than 50% of the transactivation domain, indicating that aberrant Pax5 production might lead to the arrest of B-cell differentiation, contributing to the pathogenesis of B-lineage ALL.

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