Human Fanconi A cells are susceptible to TRAIL-induced apoptosis

Authors


Carlo Dufour, M.D, Haematology Unit, Department of Paediatric Haemato-Oncology, G. Gaslini Institute, Largo G. Gaslini 5, 16148 Genova, Italy.
E-mail: carlodufour@ospedale-gaslini.ge.it

Summary

Tumour necrosis factor (TNF) contributes to the pathogenesis of bone marrow failure in Fanconi anaemia (FA) patients. The sensitivity of haematopoietic cells from FA, complementation group A (FANCA) subjects, who represent the majority of FA patients, to TNF-related apoptosis-inducing ligand (TRAIL) is unknown. The human lymphoblastoid FANCA HSC072 cell line and the genetically corrected counterpart HSC072FANCA-neo were tested for apoptoptic response to TRAIL using flow cytometry and Western blotting. FANCA cells were more sensitive to TRAIL-induced apoptosis than their corrected counterparts, indicating that TRAIL negatively regulates haematopoietic FANCA cell lines. This effect involved poly(ADP-ribose) polymerase-1 cleavage and caspase-8 activation.

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