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Hereditary thrombophilia is associated with an increased risk of fetal loss. Assuming that fetal loss is due to placental thrombosis, anticoagulant treatment might improve pregnancy outcome. In an observational family cohort study, we prospectively assessed the effects of anticoagulant drugs on fetal loss rates in women with hereditary deficiencies of antithrombin, protein C or protein S. The cohort contained 376 women (50 probands and 326 deficient or non-deficient relatives). Probands were consecutive deficient patients with venous tromboembolism. Thromboprophylaxis during pregnancy was recommended in deficient women, irrespective of prior venous thromboembolism, and in non-deficient women with prior venous thromboembolism. Outcome of first pregnancy was analysed in 55 eligible women. Of 37 deficient women, 26 (70%) received thromboprophylaxis during pregnancy, compared with three of 18 (17%) non-deficient women. Fetal loss rates were 0% in deficient women with thromboprophylaxis versus 45% in deficient women without (P = 0·001) and 7% in non-deficient women without thromboprophylaxis (P = 0·37). The adjusted relative risk of fetal loss in women who received thromboprophylaxis versus women who did not was 0·07 (95% confidence interval 0·001–0·7; P = 0·02). Our data suggest that anticoagulant treatment during pregnancy reduces the high fetal loss rate in women with hereditary deficiencies of antithrombin, protein C or protein S.
Fetal loss is a major problem in human pregnancy. Thrombosis of the uteroplacental vessels is one of the pathophysiological mechanisms that are presumed to cause fetal loss (Rai et al, 1996). Changes in blood coagulation and fibrinolysis during normal pregnancy induce a state of hypercoagulability, which predisposes to the development of thrombosis (Stirling et al, 1984). Hereditary thrombophilic defects are associated with an increased risk of venous thromboembolism (Bertina et al, 1994; Poort et al, 1996; Sanson et al, 1999). In combination with the physiological changes during pregnancy, these defects may increase the risk of uteroplacental thrombosis and hence the risk of fetal loss (Kupferminc, 2003). Although the results from previous studies are contradictory, there is evidence of an increased risk of fetal loss associated with various thrombophilic defects, including hereditary deficiencies of antithrombin, protein C and protein S, factor V Leiden and prothrombin G20210A (Sanson et al, 1996; Meinardi et al, 1999; Foka et al, 2000)
Considering uteroplacental thrombosis as a cause of fetal loss, it is rational to use anticoagulant drugs for its prevention. A benefit of these drugs might particularly be expected in women with deficiencies of antithrombin, protein C or protein S, as these thrombophilic defects are strong risk factors for thrombosis (Sanson et al, 1999). Of four previously reported studies (Brenner et al, 2000; Kupferminc et al, 2001; Gris et al, 2004; Vossen et al, 2004), three suggested an improved pregnancy outcome in women with various thrombophilic defects, who received anticoagulant treatment during pregnancy (Brenner et al, 2000; Kupferminc et al, 2001; Gris et al, 2004).
We performed a prospective, observational study to asses the effects of anticoagulant drug treatment during pregnancy on the risk of fetal loss in a cohort of women with hereditary deficiencies of either antithrombin, protein C or protein S.
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- Materials and methods
Overall, of 50 female probands and 326 female relatives, 286 were eligible i.e. alive and 15 years of age or older (Table I). Consent was refused or not obtained due to geographic reasons in 12 relatives (response rate 96%) and 219 women were excluded because they had no pregnancies (218) or only terminated pregnancies (1). The remaining 55 women were evaluable, of whom 37 were deficient and 18 non-deficient. Their characteristics are summarised in Table II. Fourteen of 37 deficient women and eight of 18 non-deficient women had been pregnant and two women in each group had fetal loss prior to enrolment. Venous thromboembolism had occurred prior to enrolment in 57% of deficient women, compared with 6% of non-deficient women (P < 0·001).
Table I. Recruitment of study population.
| ||Antithrombin-deficient families (n = 12)||Protein C-deficient families (n = 40)||Protein S-deficient families (n = 39)||Total (n = 91)|
| Geographic reasons||1||2||0||3|
|Only terminated pregnancies||0||1||0||1|
| Protein levels, IU/dl; mean (SD)||55 (8)||55 (16)||50 (9)||53 (11)|
| Protein levels, IU/dl; mean (SD)||95 (8)||100 (18)||84 (7)||96 (15)|
Table II. Characteristics of women with hereditary deficiencies of either antithrombin, protein C or protein S and their non-deficient female relatives.
| ||Deficient (n = 37)||Non-deficient (n = 18)|| P-value|
|Prior to enrolment|
| Pregnant, n (%)||14 (38)||8 (44)||0·8|
| Fetal loss, n (%)||2 (5)||2 (11)||0·6|
| Venous thromboembolism, n (%)||21 (57)||1 (6)||<0·001|
| Age at enrolment, median (range), years||30 (18–37)||25 (15–47)||0·1|
| Follow-up period, median (range), years||7·6 (0·5–24)||8·5 (0·07–24)||0·8|
| Age at first pregnancy, median (range), years||29 (21–37)||27 (16–33)||0·06|
| Thromboprophylaxis, n (%)||26 (70)||3 (17)||<0·001|
| LMW heparin, n||10||1|| |
| UF heparin and vitamin K antagonists, n||2||1|| |
| LMW heparin and vitamin K antagonists, n||14||1|| |
Of 37 deficient women, 26 (70%) received thromboprophylaxis during pregnancy (18/21 with previous venous thromboembolism and 8/16 without a history of venous thromboembolism), compared with three of 18 (17%) non-deficient women (one with a history of venous thromboembolism and two without an obvious reason for thromboprophylaxis) (Table II). Thromboprophylaxis was refused by 11 deficient women; eight had no history of venous thromboembolism and nine had not been pregnant before. Median age (range) at first pregnancy was 23 years (16–31 years) in these women, compared with 28 years (19–33 years) in deficient women who received thromboprophylaxis (P = 0·04). In non-deficient women, the median age was 26 years (21–30 years) (with thromboprophylaxis) versus 29 years (22–37 years) (without thromboprophylaxis).
Sixty-nine per cent of all women had one or more other thrombophilic defects. These included prothrombin G20210A in 8% of deficient versus 0% of non-deficient women, factor V Leiden in 19% vs. 14%, increased factor VIII levels in 43% versus 62%, increased factor IX levels in 9% vs. 7%, increased factor XI levels in 3% vs. 7% and hyperhomocysteinaemia in 12% vs. 15%. The prevalences of these defects were higher in both deficient and non-deficient women than expected in the normal population, except for high factor IX and XI levels in deficient and non-deficient women, and prothrombin G20210A in non-deficient women (expected: prothrombin G20210A 1–2%; factor V Leiden 5%; increased factor VIII 25%; increased factor IX and factor XI, and hyperhomocysteinaemia, each 10%). None of the differences were statistically significant. Thrombophilic defects were equally distributed among subgroups of women who received thromboprophylaxis or did not (data not shown). Lupus anticoagulant was not found in deficient or non-deficient women.
None of 26 deficient women (0%) with thromboprophylaxis experienced fetal loss, in contrast to five of 11 deficient women (45%) without thromboprophylaxis (P = 0·001) (Table III). Fetal loss rates were comparable in deficient women with thromboprophylaxis (0%) and non-deficient women without thromboprophylaxis (7%) (P = 0·37). The relative risk of fetal loss in women who received thromboprophylaxis compared to women without thromboprophylaxis, adjusted for clustering of women in families, was 0·07 [95% confidence interval (CI), 0·01–0·7]. Fetal loss rates when women with and without thromboprohylaxis were compared, were: 0% vs. 63% in antithrombin-deficient women; 0% vs. 50% in protein C-deficient women; and 8% vs. 57% in protein S-deficient women. In all cases, fetal loss occurred before 22 weeks of gestation.
Table III. Fetal loss in deficient and non-deficient women, who did or did not receive thromboprophylaxis during pregnancy.
| Fetal loss, n (%)||1 (2)||11 (58)||1 (25)||1 (5)|
| Fetal loss, n (%)*||0 (0)||5 (45)||1 (33)||1 (7)|
| ||P = 0·001†||P = 0·37†|
Three women experienced venous thromboembolism related to the first pregnancy after enrolment. All were antithrombin-deficient and had received thromboprophylaxis. Two events were recurrences of venous thromboembolism. Only one woman, who was protein S-deficient, experienced pregnancy-induced hypertension. Complications related to the use of anticoagulants (major bleeds) were not observed.
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- Materials and methods
Our study showed that thromboprophylaxis resulted in a 15-fold reduction in fetal loss rate. In deficient women on thromboprophylaxis, fetal loss rate was significantly lower than in deficient women without prophylaxis (0% vs. 45%, P = 0·001), while it was comparable with the fetal loss rate in non-deficient women without prophylaxis (0% vs. 7%, P = 0·37). The number of non-deficient women on thromboprophylaxis was too small to allow analysis.
Thus far, four studies addressed the effects of anticoagulants on the outcome of pregnancy in women with various thrombophilic defects (Brenner et al, 2000; Kupferminc et al, 2001; Gris et al, 2004; Vossen et al, 2004). In the first study, 50 women with unexplained fetal loss and single or combined thombophilic defects [protein S deficiency, factor V Leiden, prothrombin G20210A and methylenetetrahydrofolate reductase (MTHFR)] received LMW heparin during their next pregnancy (Brenner et al, 2000). The fetal loss rate dropped to 25%. In the second study, 33 women with a history of either fetal loss, pre-eclampsia, abruptio placentae or intrauterine growth retardation, and a thrombophilic defect (protein S deficiency, factor V Leiden, prothrombin G20210A or MTHFR), were treated with a prophylactic dose of LMW heparin and low dose aspirin (100 mg/d) during the next pregnancy (Kupferminc et al, 2001). Recurrent events in the next pregnancy were reported in only 9% of the women. The third study compared a prophylactic dose of LMW heparin to low dose aspirin in 160 women with one prior unexplained fetal loss after 10 weeks of gestation and a thrombophilic defect (protein S deficiency, factor V Leiden or prothrombin G20210A) (Gris et al, 2004). Fetal loss rates in this randomised trial, without an untreated or placebo-treated control group, were 14% and 77% in women who were treated with LMW heparin and aspirin respectively. No benefit of anticoagulant treatment was demonstrated in the fourth study (Vossen et al, 2004). Twenty-one women with a deficiency of antithrombin, protein C or protein S or factor V Leiden, received heparin or vitamin K antagonists. Indications for this treatment were venous thromboprophylaxis, prevention of fetal loss or unknown. Fetal loss rates were 24% in women with anticoagulant treatment compared with 27% in controls (adjusted relative risk 0·7: 95% CI, 0·2–3·2).
A comparison of these studies with our study is difficult, due to differences in design, selection criteria and thrombophilic defects. The women in our study were not selected for a compromised obstetric history. We recommended treatment with high doses of anticoagulants during pregnancy and puerperium in all deficient women, considering that they were at high risk of venous thromboembolism. This assumption was confirmed by our finding that 57% of deficient women already had experienced venous thromboembolism before they were enroled at a median age of 30 years, compared with 6% of non-deficient women. Moreover, 5% of deficient women had pregnancy-related venous thromboembolism after enrolment despite thromboprophylaxis. For the same reason, a randomised placebo-controlled clinical trial to assess the effects of anticoagulant drug therapy on fetal loss was, in our opinion, unethical. Nevertheless, one-third of deficient women preferred not to be treated with anticoagulants. As a majority of these women, who were of younger age, had no history of venous thromboembolism, their reluctance to thromboprophylaxis was understandable. However, they showed an excessively high fetal loss rate (45%), suggesting that deficient women are at high risk of both venous thromboembolism and fetal loss. A possible explanation for the high risk is the concomitance of one or more other thrombophilic defects, observed in 69% of deficient and non-deficient women. Single or multiple mild thrombophilic defects, in addition to hereditary deficiencies of antithrombin, protein C and protein S, known as strong thrombotic risk factors, might increase the risk of fetal loss due to placental thrombosis, as we previously demonstrated for the risk of venous thromboembolism (Brouwer et al, 2006).
Small numbers of women did not allow reliable estimates of fetal loss rates associated with specific combinations of deficiencies and concomitant thrombophilic defects. As concomitant thrombophilic defects were equally distributed among deficient women who received thromboprophylaxis and deficient women who did not, it is likely that the difference in fetal loss rates would be attributed to thromboprophylaxis.
Other causes of fetal loss, like abnormal karyotype, congenital anomalies and uterine malformation were not investigated. However, these were probably equally distributed among women on thromboprophylaxis and women who did not receive thromboprophylaxis, while an effect of thromboprophylaxis on these conditions is not plausible.
Small numbers of women and a non-randomised design are the main limitations of this study. Whether a randomised clinical trial on fetal loss is feasible in women who are at high risk of venous thromboembolism, remains a matter of debate. It may be easier to perform such a clinical trial in women with more prevalent, mild thrombophilic defects. However, as the risk of venous thromboembolism, as well fetal loss is lower in these women, the potential benefit of anticoagulant drug therapy, and consequently its clinical impact, may be limited.
In conclusion, our data suggest that strong thrombophilic defects, like hereditary deficiencies of antithrombin, protein C and protein S, and combinations of these deficiencies with other, mild thrombophilic defects, are associated with a high risk of fetal loss. This risk seems to be reduced by anticoagulant drug therapy during pregnancy.