Mesenchymal content of fresh bone marrow: a proposed quality control method for cell therapy
Article first published online: 25 SEP 2007
British Journal of Haematology
Volume 139, Issue 2, pages 312–320, October 2007
How to Cite
Veyrat-Masson, R., Boiret-Dupré, N., Rapatel, C., Descamps, S., Guillouard, L., Guérin, J.-J., Pigeon, P., Boisgard, S., Chassagne, J. and Berger, M. G. (2007), Mesenchymal content of fresh bone marrow: a proposed quality control method for cell therapy. British Journal of Haematology, 139: 312–320. doi: 10.1111/j.1365-2141.2007.06786.x
- Issue published online: 25 SEP 2007
- Article first published online: 25 SEP 2007
- Received 27 April 2007; accepted for publication 15 June 2007
- non-expanded (native) mesenchymal stem cell;
- bone marrow;
- cell therapy;
- quality control
The scarcity of mesenchymal stem cells (MSC) in bone marrow (BM) has justified their ex vivo expansion before therapeutic use, but a method to evaluate the quality of initial mesenchymal content and track the modifications induced by graft processing has not yet been proposed. The aim of this study was to establish such a procedure. Flow cytometric and functional assay methods were modified to count CD45− CD14−/CD73+ subsets containing all MSC and used them to study BM from spongy bone (SB) and iliac crest aspirate (ICA). These methods detected the target subsets in all BM suspensions derived from SB (n = 154) and ICA, (n = 44) with a satisfactory correlation between immuno-phenotyping and functional tests by low-density plating. We noted a higher overall MSC frequency in SB cell suspensions but a lower plating efficiency of CD45− CD14−/CD73+ SB cells under standard culture conditions.
We propose a cell quality control on un-manipulated BM cell suspensions to quantify the mesenchymal compartment with regard to varying donor factors, such as age and sampling site, that influence expansion and define a therapeutic threshold value. Furthermore, we were able to confirm differences in plating efficiency and proliferative capacity between two BM origins.