These authors contributed equally to this work.
B-cell chronic lymphocytic leukaemia cells show specific changes in membrane protein expression during different stages of cell cycle
Article first published online: 29 OCT 2007
British Journal of Haematology
Volume 139, Issue 4, pages 600–604, November 2007
How to Cite
Bennett, F., Rawstron, A., Plummer, M., Tute, R. d., Moreton, P., Jack, A. and Hillmen, P. (2007), B-cell chronic lymphocytic leukaemia cells show specific changes in membrane protein expression during different stages of cell cycle. British Journal of Haematology, 139: 600–604. doi: 10.1111/j.1365-2141.2007.06790.x
- Issue published online: 29 OCT 2007
- Article first published online: 29 OCT 2007
- Received 26 May 2007; accepted for publication 11 July 2007
- chronic lymphocytic leukaemia;
The proliferating component in chronic lymphocytic leukaemia (CLL) is usually small (<1%) and restricted to a specific micro-environmental niche. To characterize the proliferating component, CLL cells from bone marrow or lymph nodes of 23 patients were assessed for expression of up to 66 surface antigens in combination with nuclear Ki-67/MCM6. Ki-67 expression was associated with step-wise increases in CD23/CD95/CD86/CD39/CD27 and decreases in CD24/CD69/CXCR4/CXCR5. Ki-67+ cells showed increased CD38 expression, but with considerable inter-patient variability: in some cases Ki-67 expression was only detectable in CD38− CLL cells. The results suggest continuous re-entry into the cell cycle as no distinct stem cell pool was detectable.