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Fig S1. Treatment with MS-1020 has no effect on Tyk2 phosphorylation. U266 cells were cultured for 16 h in the presence of either vehicle (DMSO) alone, MS-1020 at different concentrations or the JAK kinase inhibitor AG490 (150 μmol/l), and then stimulated with 1000 U/ml IFN-α for 30 min. Whole-cell extracts were processed for Western blot analysis using phospho-Tyk2 and Tyk2 antibodies. GAPDH serves as a loading control.

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