Immunotoxin BL22 induces apoptosis in mantle cell lymphoma (MCL) cells dependent on Bcl-2 expression
Version of Record online: 11 OCT 2009
© 2009 Blackwell Publishing Ltd
British Journal of Haematology
Volume 148, Issue 1, pages 99–109, January 2010
How to Cite
Bogner, C., Dechow, T., Ringshausen, I., Wagner, M., Oelsner, M., Lutzny, G., Licht, T., Peschel, C., Pastan, I., Kreitman, R. J. and Decker, T. (2010), Immunotoxin BL22 induces apoptosis in mantle cell lymphoma (MCL) cells dependent on Bcl-2 expression. British Journal of Haematology, 148: 99–109. doi: 10.1111/j.1365-2141.2009.07939.x
- Issue online: 14 DEC 2009
- Version of Record online: 11 OCT 2009
- Received 31 March 2009; accepted for publication 25 August 2009
Fig S1. (A) NCEB-1, NCEB-1/t BCL-2 cells, JeKo-1, JeKo-1/t-BCL2 cells as well as JVM-2 and JVM-2/tBCL2 cells were cultured in the presence of BL22 at the indicated concentration for 48 h. Phosphatidylserine exposure was measured using the annexin V assay. The mean percentage of viable cells (annexin – and PI- negative) ± SEM of three different experiments is shown. Paired t-tests were performed to test for differences between NCEB-1 and NCEB-1/tBCL2 cells (*), JeKo-1 and JeKo-1/tBCL2 cells (#), as well as JVM-2 and JVM-2/tBCL2 cells (+). */#/+ indicates when the difference between the cells was statistically significant (P < 0.05) (*: P 0.0217), (#: P 0.0175), (+: P 0.0408). (B) Bcl-2 expression in JVM-2 cells measured via FACS analysis. (C) Representative data of a CD22 expression analysis for JeKo-1, NCEB-1 and JVM-2 cells. Also shown are the isotype controls.
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