Interferon affects cell growth progression by modulating DNA polymerases activity

  1. A. Cataldi1,
  2. E. Santavenere1,
  3. M. Vitale2,
  4. O. Trubiani3,
  5. R. Lisio3,
  6. G. Tulipano1,
  7. L. Di Domenicantonio3,
  8. L. Zamai4,
  9. S. Miscia1,*

Article first published online: 1 MAY 2008

DOI: 10.1111/j.1365-2184.1992.tb01397.x

Issue

Cell Proliferation

Cell Proliferation

Volume 25, Issue 3, pages 225–231, May 1992

Additional Information

How to Cite

Cataldi, A., Santavenere, E., Vitale, M., Trubiani, O., Lisio, R., Tulipano, G., Di Domenicantonio, L., Zamai, L. and Miscia, S. (1992), Interferon affects cell growth progression by modulating DNA polymerases activity. Cell Proliferation, 25: 225–231. doi: 10.1111/j.1365-2184.1992.tb01397.x

Author Information

  1. 1

    Istituto di Morfologia Umana Normale, Chieti

  2. 2

    *Istituto di Anatomia Umana Normale, Bologna

  3. 3

    †Istituto di Citomorfologia Normale e Patologica del C.N.R., Chieti

  4. 4

    ‡Lab. Biol. Cell. e Microscopia Elettronica I.O.R., Bologna, Italy

* Istituto di Morfologia Umana Normale, Via dei Vestini 6, 66100 Chieti, Italy

Publication History

  1. Issue published online: 1 MAY 2008
  2. Article first published online: 1 MAY 2008
  3. Received 22 July 1991; revision accepted 4 November 1991

SEARCH

SEARCH BY CITATION

ARTICLE TOOLS

Get PDF (343K)

Abstract. A multiparametric analysis of the effects of human recombinant interferon alpha type A on Daudi cells involving flow cytometry and in vitro analysis of alpha and beta DNA polymerase activities has been performed. Results have disclosed (within 60 min of interferon treatment) a decrease of alpha polymerase driven DNA synthesis persisting to at least 24 h, while beta polymerase was poorly affected. Moreover, after 24 h of interferon treatment, a reduction of BrdUrd incorporation per cell, assessed by flow cytometry, was observed suggesting that DNA synthesis in S phase cells is almost completely abolished. The analysis of the effect of interferon on the distribution of cell cycle phases indicated that the G1/S transition is not inhibited by the treatment. These results support the hypothesis that interferon generates a transient initiating signal which quickly reaches the nucleus and produces a rapid inhibition of alpha polymerase activity, leading finally to the slowing of cell cycle progression.

Get PDF (343K)