Effects of IL-11 on the growth of intestinal epithelial cells in vitro

Authors

  • C. Booth,

    Corresponding author
    1. CRC Department of Epithelial Biology, Paterson Institute for Cancer Research, Christie Hospital (NHS) Trust, Withington, Manchester, UK
      CRC Department of Epithelial Biology, Paterson Institute of Cancer Research, Christie Hospital (NHS) Trust, Withington, Manchester M20 9BX, UK.
    Search for more papers by this author
  • C. S. Potten

    1. CRC Department of Epithelial Biology, Paterson Institute for Cancer Research, Christie Hospital (NHS) Trust, Withington, Manchester, UK
    Search for more papers by this author

CRC Department of Epithelial Biology, Paterson Institute of Cancer Research, Christie Hospital (NHS) Trust, Withington, Manchester M20 9BX, UK.

Abstract

The network of interacting factors that control proliferation in the intestinal epithelium is largely unknown. Recently, IL-11 was found to protect animals from lethal doses of cytotoxic agents. Part of this protective action was ascribed to a reduced level of damage in the intestinal epithelium. Whether this was due to a direct effect on epithelial cell cycle progression was unclear. We have addressed this question in vitro and found that IL-11 reversibly inhibited proliferation in untransformed small intestinal IEC18 cells. However, IL-11 did not inhibit transformed SW620 or HT29 colonic cell lines. IL-6 behaved in a similar manner to IL-11. Thus, these results suggest that IL-11 may be an ideal therapy adjuvant, protecting normal cells and further, these results suggest that IL-11 may be involved in the normal growth controls in the intestinal epithelium. The inhibitory response evoked by IL-11 is lost during carcinogenic transformation.

Ancillary