Long-term proliferation and characterization of human spermatogonial stem cells obtained from obstructive and non-obstructive azoospermia under exogenous feeder-free culture conditions
Article first published online: 29 JUN 2010
© 2010 Blackwell Publishing Ltd
Volume 43, Issue 4, pages 405–417, August 2010
How to Cite
Lim, J. J., Sung, S.-Y., Kim, H. J., Song, S.-H., Hong, J. Y., Yoon, T. K., Kim, J. K., Kim, K.-S. and Lee, D. R. (2010), Long-term proliferation and characterization of human spermatogonial stem cells obtained from obstructive and non-obstructive azoospermia under exogenous feeder-free culture conditions. Cell Proliferation, 43: 405–417. doi: 10.1111/j.1365-2184.2010.00691.x
- Issue published online: 29 JUN 2010
- Article first published online: 29 JUN 2010
- Received 8 September 2009; revision accepted 26 November 2009
Objectives: The aim of the present study was to improve efficiency of isolation and to optimize proliferative potential of human spermatogonial stem cells (SSCs) obtained from obstructive azoospermic (OA) and non-obstructive azoospermic (NOA) patients, and further, to characterize these cells for potential use in infertility treatment or study of reproductive biology.
Materials and methods: We have applied a cell-sorting method, using collagen and magnetic activated cell separation to overcome obstacles, developing a collection system, and simple long-term proliferation system, that yields large numbers of high-purity SSCs from obstructive OA and NOA patients.
Results: SSCs derived from OA and NOA patients proliferated and maintained their characteristics for more than 12 passages (>6 months) in vitro. Moreover, the population of cells positive for the SSC-specific markers GFRα-1 and integrin α6, increased to more than 80% at passage 8.
Conclusion: These finding may support the idea that in vitro propagation of SSCs could be a useful tool for infertility treatment and study of reproductive biology.