The first two authors contributed equally to this work.
Up-regulation of Anxa2 gene promotes proliferation and invasion of breast cancer MCF-7 cells
Article first published online: 27 MAR 2012
© 2012 Blackwell Publishing Ltd
Volume 45, Issue 3, pages 189–198, June 2012
How to Cite
Wu, B., Zhang, F., Yu, M., Zhao, P., Ji, W., Zhang, H., Han, J. and Niu, R. (2012), Up-regulation of Anxa2 gene promotes proliferation and invasion of breast cancer MCF-7 cells. Cell Proliferation, 45: 189–198. doi: 10.1111/j.1365-2184.2012.00820.x
- Issue published online: 17 APR 2012
- Article first published online: 27 MAR 2012
- Manuscript Accepted: 22 FEB 2012
- Manuscript Received: 27 NOV 2011
- National Natural Science Foundation of China. Grant Number: 81071731
- NSFC. Grant Number: 81001188
- Tianjin Higher Education Science & Technology Fund Planning Project. Grant Number: 20100120
The metastatic ability of breast cancer cells with chemoresistant properties is higher when compared to that of their parental wild-type cells. Expression of AnnexinA2 (Anxa2), a 36-kDa calcium-dependent phospholipid binding protein, is increased in metastatic tumours and has been found to be associated with the phenotype of drug resistance and metastasis.
Materials and Methods and Results
In the present study, we found that up-regulation of Anxa2 correlates with enhanced migration and invasion ability of MCF-7 breast cancer cells both in vitro and in vivo. Western blot analysis revealed that exposure to chemotherapeutic drugs may induce elevated expression of Anxa2. In addition, our data have shown that Anxa2 might influence proliferation, migration and invasion of MCF-7 cells by increasing expression of c-myc and cyclin D1 via activation of Erk1/2 signalling pathways.
Our findings suggest that up-regulation of Anxa2 may play an important role in modulating proliferation and invasion of breast cancer MCF-7 cells through regulation of many relevant downstream target genes.